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Changes in Synapsin Levels in the Millipede Gymnostreptus olivaceus Schubart, 1944 Exposed to Different Concentrations of Deltamethrin

Published online by Cambridge University Press:  08 January 2016

Annelise Francisco ,
Pablo H. Nunes ,
Roberta C. F. Nocelli  and
Carmem S. Fontanetti
Annelise Francisco
Affiliation:
Departamento de Biologia, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista (UNESP), Bela Vista, 13.500-900, Rio Claro, São Paulo, Brazil
Pablo H. Nunes
Affiliation:
Centro Interdisciplinar de Ciências da Vida e da Natureza (CICV), Instituto Latino-Americano de Ciências da vida e da Natureza (ILACVN), Universidade Federal da Integração Latino-Americana (UNILA), Avenida Silvio Américo Sasdelli, 1842, 85.866-000, Foz do Iguaçu, Paraná, Brazil
Roberta C. F. Nocelli
Affiliation:
Departamento de Ciências da Natureza, Matemática e Educação, Centro de Ciências Agrárias, Universidade Federal de São Carlos (UFSCar), Via Anhanguera, Km 174, 13.600-970, Araras, São Paulo, Brazil
Carmem S. Fontanetti*
Affiliation:
Departamento de Biologia, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista (UNESP), Bela Vista, 13.500-900, Rio Claro, São Paulo, Brazil
*
*Corresponding author.[email protected]
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Abstract

Millipedes are ecologically important soil organisms and may also be an economically threatening species in rural and urban areas when population outbreaks occur. In order to control infestations commercial formulations of deltamethrin have been commonly applied, even though there are few studies about the effects of such insecticide on millipedes. This paper describes the effects of this insecticide on millipedes showing neurotoxic effects assessed by synapsin labeling and confocal microscopy. Deltamethrin concentrations related to the DL50 of the active ingredient and a field concentration were applied topically in the diplopod Gymnostreptus olivaceus to evaluate the behavior, mortality rate, and synapsin levels in the brain 12, 24, and 48h after contact with deltamethin. The insecticide caused mortality at the higher concentrations employed, in which no change was observed in neurotransmission in the survivors. In contrast, at field concentrations, deltamethrin did not cause any deaths, but triggered significant changes in synapsin levels. The results obtained form the synapsin labeling provide several interpretations suggesting that the isolated application of this tool must be associated with additional tools in order to evaluate biologically induced effects of deltamethrin in an accurate way. In addition, the feasibility of chemical control of millipedes with deltamethrin is questioned.

Keywords

arthropodconfocal microscopydiplopodolfactory glomerulipyrethroid
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 1 , February 2016 , pp. 48 - 54
Copyright
© Microscopy Society of America 2016 

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