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Centrosome Fine Ultrastructure of the Osteocyte Mechanosensitive Primary Cilium

Published online by Cambridge University Press:  21 November 2012

R.E. Uzbekov ,
D.B. Maurel ,
P.C. Aveline ,
S. Pallu ,
C.L. Benhamou  and
G.Y. Rochefort
R.E. Uzbekov*
Affiliation:
Department of Microscopy, François Rabelais University, Tours, France Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia
D.B. Maurel
Affiliation:
EA4708 I3MTO, Orléans University, IPROS, Hospital Porte Madeleine, Orleans, France
P.C. Aveline
Affiliation:
EA4708 I3MTO, Orléans University, IPROS, Hospital Porte Madeleine, Orleans, France
S. Pallu
Affiliation:
EA4708 I3MTO, Orléans University, IPROS, Hospital Porte Madeleine, Orleans, France
C.L. Benhamou
Affiliation:
EA4708 I3MTO, Orléans University, IPROS, Hospital Porte Madeleine, Orleans, France
G.Y. Rochefort*
Affiliation:
EA4708 I3MTO, Orléans University, IPROS, Hospital Porte Madeleine, Orleans, France
*
*Corresponding author. E-mail: [email protected]
**Corresponding author. E-mail: [email protected]
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Abstract

The centrosome is the principal microtubule organization center in cells, giving rise to microtubule-based organelles (e.g., cilia, flagella). The aim was to study the osteocyte centrosome morphology at an ultrastructural level in relation to its mechanosensitive function. Osteocyte centrosomes and cilia in tibial cortical bone were explored by acetylated alpha-tubulin (AαTub) immunostaining under confocal microscopy. For the first time, fine ultrastructure and spatial orientation of the osteocyte centrosome were explored by transmission electron microscopy on serial ultrathin sections. AαTub-positive staining was observed in 94% of the osteocytes examined (222/236). The mother centriole formed a short primary cilium and was longer than the daughter centriole due to an intermediate zone between centriole and cilium. The proximal end of the mother centriole was connected with the surface of daughter centriole by striated rootlets. The mother centriole exhibited distal appendages that interacted with the cell membrane and formed a particular structure called “cilium membrane prolongation.” The primary cilium was mainly oriented perpendicular to the long axis of bone. Mother and daughter centrioles change their original mutual orientation during the osteocyte differentiation process. The short primary cilium is hypothesized as a novel type of fluid-sensing organelle in osteocytes.

Keywords

osteocytecentrosomecentrioleprimary ciliumstriated rootletsmechanotransductiontransmission electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 6 , December 2012 , pp. 1430 - 1441
Copyright
Copyright © Microscopy Society of America 2012

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