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Biological Relevancy of Quantitative Analysis of Adherence Receptors on Polymorphonuclear.Neutrophil by Flow Cytometry and Optical Scanning Microscopy..

Published online by Cambridge University Press:  02 July 2020

Dominique Dumas ,
Véronique Latger  and
Jean F. Stoltz
Dominique Dumas
Affiliation:
Laboratoire d’Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les-Nancy, France., [email protected].
Véronique Latger
Affiliation:
Laboratoire d’Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les-Nancy, France., [email protected].
Jean F. Stoltz
Affiliation:
Laboratoire d’Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les-Nancy, France., [email protected].
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Extract

Leukocytes adherence receptors are the basis of cells interactions1. Flow cytometry (FCM) makes it possible to assess a characterization of the activation level of a given cellular population by receptor quantifying2. That technique, however does not integrate at the same time, all others factors of leucocyte adhesive phenotype regulation, as spatial distribution and molecular conformation. Our study consisted in exploring the main adhesion receptors (CD62L, CDllb/CD18) on the surface of Polymorphonuclear Neutrophils (PMN) that were prepared under identical conditions and simultaneously analyzed by FCM and Conventional Optical Scanning Microscopy/Deconvolution3 (COSM). Methods: PMN cells from whole blood were obtained using Polymorphprep™. Isolated PMN (105 cells /ml) were incubated with Tumor Necrosis Factor (TNFα, 100 UI/ml) at 37°C for one hour. Quantification of cell surface molecules was assessed according to the quantitative indirect immunofluorescence3 method using QIFIKIT® (Dako, France).

Type
Blood/Immunology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1122 - 1123
Copyright
Copyright © Microscopy Society of America

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References

1.)Brown, E.J. Cell Biology. 7 (1997)289.Google Scholar
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4.)Poncelet, P. and Carayon, P.. J. Immunol Methods. 85(1985)65.CrossRefGoogle Scholar