Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T11:39:33.005Z Has data issue: false hasContentIssue false
  • English
  • Français

Automated Acquisition of Cryo-Electron Microscope Images With Slow-Scan CCD Cameras and Microscope Control

Published online by Cambridge University Press:  02 July 2020

M. Pan ,
N.H. Olsont  and
T.S. Bakert
M. Pan
Affiliation:
Gatan, R&D, 6678 Owens Drive, Pleasanton, CA94588
N.H. Olsont
Affiliation:
Department of Biological Sciences, Purdure University, West Lafayette, IN47907
T.S. Bakert
Affiliation:
Department of Biological Sciences, Purdure University, West Lafayette, IN47907
Get access

Extract

Recording electron microscope images of frozen-hydrated samples under cryo-conditions is a difficult and challenging task. The inherently low sample contrast makes accurate focusing difficult. Furthermore most frozen-hydrated samples are very beam sensitive so that electron dose on the sample must be kept to the minimum. In recent years, the new generation of cooled slow-scan CCD (SSC) cameras have shown superior properties in sensitivity, resolution, dynamic range, linearity, and more importantly their ability to display images on-line. Computer control of electron microscopes via the standard RS232C serial interface has been greatly facilitated by the use of on-line CCD images. We have developed a software package that allows cryo-electron microscope images to be automatically acquired onto a CCD camera via computer control of the microscope. This fully automated feature allows quality cryo-electron microscope images to be recorded with increased efficiency and enhances the possibility of obtaining three-dimensional reconstructions from images.

Type
Computational Advances and Enabling Technologies for 3D Microscopies in Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 1113 - 1114
Copyright
Copyright © Microscopy Society of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Dubochet, J.et al., Quart. Rev. Biophys. 21(1988) 129.10.1017/S0033583500004297CrossRefGoogle Scholar
2.Krivanek, O.L. and Mooney, P.E., Ultramicroscopy 49 (1993) 95.10.1016/0304-3991(93)90216-KCrossRefGoogle Scholar
3.Pan, M.et al., (this proceeding).Google Scholar
4.Bloom, M.E.et al., J. Virol. 35(1980) 836.10.1128/JVI.35.3.836-843.1980CrossRefGoogle Scholar
5. This study was funded in part by an NSF shared equipment grant (BIR 9112921) and NIH grants (GM33050 and AI35212) to T.S.B.Google Scholar