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Atomic Force Microscopy Study of the Anti-inflammatory Effects of Triptolide on Rheumatoid Arthritis Fibroblast-like Synoviocytes

Published online by Cambridge University Press:  26 July 2017

Zhanhui Su ,
Han Sun ,
Man Ao  and
Chunying Zhao
Zhanhui Su*
Affiliation:
Hebei Key Laboratory of Research and Development for Traditional Chinese Medicine, Institute of Chinese Materia Medica, Chengde Medical College, Chengde 067000, China
Han Sun
Affiliation:
Chengde Nursing Vocational College, Chengde 067000, China
Man Ao
Affiliation:
Affiliated Hospital of Chengde Medical College, Chengde 067000, China
Chunying Zhao
Affiliation:
Hebei Key Laboratory of Research and Development for Traditional Chinese Medicine, Institute of Chinese Materia Medica, Chengde Medical College, Chengde 067000, China
*
*Corresponding author. [email protected]
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Abstract

High-resolution atomic force microscopy (AFM) was used for the in situ evaluation of the anti-inflammatory effects of triptolide on rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) to understand the anti-RA effects of triptolide, based on the morphological and biophysical changes observed in RA-FLS. RA-FLS have been reported to play a primary role in inflammatory bone destruction during the development of RA and thus are regarded as an important target for RA treatment. Triptolide pretreatment significantly inhibited tumor necrosis factor-α-induced expression of the interleukin (IL)-1β, IL-6, and IL-8 genes in MH7A cells. Using AFM, we showed that triptolide-induced morphological damage in MH7A cells by inducing significant ultrastructure changes in the membrane, which were closely related to triptolide-induced apoptosis in MH7A cells. Using force measurements determined with AFM, triptolide was shown to increase the stiffness of MH7A cells. These findings not only revealed the strong anti-inflammatory effects of triptolide on RA-FLS, highlighting triptolide as a potential anti-RA agent, but also revealed the possible use of AFM for studying anti-inflammatory responses in RA-FLS, which we expect to be developed into a potential tool for anti-RA drug studies in RA-FLS.

Keywords

atomic force microscopymorphologystiffnesssynoviocytestriptolide
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 5 , October 2017 , pp. 1002 - 1012
Copyright
© Microscopy Society of America 2017 

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