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Aquatic Surface Film Organisms Collected On Glass Substrates For Evaluation By Scanning Electron Microscopy.

Published online by Cambridge University Press:  02 July 2020

R. W. Smith
R. W. Smith*
Affiliation:
Environmental Sciences and Resources Program-Biology, Portland State University, P.O. Box 741, Portland, Oregon97201 Oregon Health Sciences University, Department of Pathology, L-471, 3181 SW Sam Jackson Park Road, Portland, Oregon97201
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Extract

Organisms found at the Air-Water Interface (AWI) are often difficult to collect and usually show distortion upon collection and fixation by traditional methods. Furthermore, the spatial relations between the organisms and the complexity of their biofilm community is disrupted or destroyed by collection methods that involve bulk water collection, filtration, settling or rotating drums. Samples shown here were collected on the Tualatin River, Oregon, a eutrophic rural/suburban stream with welldeveloped Aquatic Surface Films (ASF or Microlayers) in quiet waters.

Here, I describe a simple method of collection for Aquatic Surface Films (Microlayers), by drawing a glass substrate slowly through the surface film, allowing both organic chemicals and small organisms to adhere to the surface of the glass. This is similar to the collection of a Langmuir-Blodgett film, and it has been proposed that chemical and biological films at the surface might allow this type of collection.

Type
Highlights Of Biological Microscopy In The Pacific Northwest Usa
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1244 - 1245
Copyright
Copyright © Microscopy Society of America

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References

1.Hardy, J. T., Progress in Oceanography, 11 (1982) 307.CrossRefGoogle Scholar
2.Smith, R. W. and J.Dash, , Proc. Ann. EMSA Meeting, 49 (1989) 1005.Google Scholar