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Applications of Confocal Microscopy to Study the Roles of the Cytoskeleton During Early Embryogenesis In Amphibians

Published online by Cambridge University Press:  02 July 2020

M. V. Danilchik ,
E. E. Brown ,
K. Larkin  and
K. Ray
M. V. Danilchik
Affiliation:
Department of Biological Structure and Function, Oregon Health Sciences University, Portland, OR97201-3097
E. E. Brown
Affiliation:
Department of Biological Structure and Function, Oregon Health Sciences University, Portland, OR97201-3097
K. Larkin
Affiliation:
Department of Biological Structure and Function, Oregon Health Sciences University, Portland, OR97201-3097
K. Ray
Affiliation:
Department of Biological Structure and Function, Oregon Health Sciences University, Portland, OR97201-3097
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Extract

Because of their great size, availability, and capacity to develop autonomously in culture, amphibian eggs have become a productive system for a wide variety of experimental studies of animal cells. Their use has advanced our understanding of fundamental molecular and cellular processes such as transcription and translation, regulation of the cell cycle, and the function of the cytoskeleton. The amphibian egg, particularly that of the frog Xenopus laevis,has further served as an excellent system for studying complex developmental phenomena, including the roles of growth factors, signal transduction, and transcription regulation in cell determination, morphogenesis, and tissue differentiation.

Despite these advantages, the frog egg's great size and opacity have made it a challenging subject for microscopy. Thus, many problems in early development, such as how maternal cytoplasmic determinants become localized during cell division, have resisted traditional optical approaches.

Type
Applications of Imaging Techniques to the Study of Embryological Development
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1076 - 1077
Copyright
Copyright © Microscopy Society of America

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References

1.Danilchik, M. V. et al, Devel. Biol. 194 (1998) 47.CrossRefGoogle Scholar
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4. This research was supported by NSF IBN-9728908 and NASA NAG2-1199.Google Scholar