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Antioxidants Stimulate Germinal Vesicle Breakdown, But Inhibit Chromosome Formation And Spindle Assembly In Porcine Oocytes

Published online by Cambridge University Press:  02 July 2020

Qing-Yuan Sun ,
Randall S. Prather  and
Heide Schatten
Qing-Yuan Sun
Affiliation:
Department of Veterinary Pathobiology, Columbia, MO65211
Randall S. Prather
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO65211
Heide Schatten
Affiliation:
Department of Veterinary Pathobiology, Columbia, MO65211
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Extract

Mammalian oocytes are arrested at the diplotene stage of the first meiotic division. Release of oocytes from their follicles induces meiotic resumption characterized by germinal vesicle breakdown (GVBD), followed by the chromosome formation and metaphase I spindle organization and finally the extrusion the first polar body. Recently it was shown that cellpermeant antioxidants significantly inhibit spontaneous resumption of meiosis in mouse oocytes, which may indicate a role of oxygen radicals in oocyte maturation. The regulation of mouse oocyte meiosis resumption is different from that of large domestic animals in that GVBD is independent of Ca2+ and protein synthesis. The present study investigated the influence of two cell-permeant antioxidants, 2(3)-ter-butyl-4-hydroxyanisole (BHA) and nordihydroguaiaretic acid (NDGA), on porcine oocyte meiosis resumption, chromatin behavior and spindle assembly. Our findings revealed a different role of antioxidants in porcine oocyte meiosis resumption than in mouse oocyte maturation.

Type
Developmental/Reproductive Biology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 964 - 965
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Takami, M. et al., Am. J. Physiol, 276 (1999) E684.Google Scholar
2.Tombes, R.M. et al., J. Cell Sci., 117 (1992) 799.Google Scholar