Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T20:43:03.710Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of Platelet Dense Granules in Storage Pool Deficient Patients

Published online by Cambridge University Press:  02 July 2020

M.M. McKenzie ,
W.T. Gunning ,
M.R. Smith ,
N.A. Lachant  and
J.N. Turner
M.M. McKenzie
Affiliation:
School of Medicine, Medical College of Ohio, Toledo, OH
W.T. Gunning
Affiliation:
Department of Pathology, Medical College of Ohio, Toledo, OH
M.R. Smith
Affiliation:
Department of Medicine, Medical College of Ohio, Toledo, OH;
N.A. Lachant
Affiliation:
Department of Medicine, Wayne State University, Detroit, MI;
J.N. Turner
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY
Get access

Extract

Prolonged bleeding times may be evident in patients with various conditions. Analysis of platelet function can provide an insight into the cause. Platelets contain substances such as ADP, calcium, epinephrine, and serotonin which are stored in the electron dense or delta granules. Our experience has shown normal platelets to have 4-6 dense granules (Figure 1), which is supported by the literature. The prolonged bleeding times of patients who have less than the normal number of dense granules may be attributed to the lack of adequate factors that aid in the clotting process. These storage pool deficient (SPD) patients are being classified by their low dense granule count. Patients with prolonged bleeding times but normal dense granule counts may need to have their granules evaluated for size and volume (Figure 2).

Whole mounted platelets were placed in the transmission electron microscope (TEM), and negatives were obtained at a magnification of 10,200x. The typical dense granule seen in normal whole mounts is circular.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 73 - 74
Copyright
Copyright © Microscopy Society of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Israels, S.J., et al.British Journal of Haematology. 75:118–121, 1990.10.1111/j.1365-2141.1990.tb02626.xCrossRefGoogle Scholar
2.Powers, L.W., et al.Diagnostic Hematology.(The C.V. Mosby Company, 1989)151.Google Scholar
3.Weiss, H.J., et al.British Journal of Haematology. 83:282–295, 1993.10.1111/j.1365-2141.1993.tb08284.xCrossRefGoogle Scholar
4.Junqueira, L.C., et al.Basic Histology. (Appleton & Lange, 1995) 233.Google Scholar