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An Introduction to Flow Cytometry in Correlative Fluorescence Studies

Published online by Cambridge University Press:  02 July 2020

R. W. Smith
R. W. Smith*
Affiliation:
Environmental Sciences and Resources Program-Biology, Portland State University, P.O. Box 751, Portland, Oregon, 97207 Oregon Health Sciences University, Department of Pathology, L-471, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201
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Abstract

In the last few years there has been a mild explosion in both microscopy and cytometry. in part, this has been a more sensitive understanding of new fluorescent chemicals or probes as well as the ability to transfect fluorescent proteins into cells. in part, this has also been a case of substantial improvement in instruments, electronics, computers, lasers and sensors. in this session, we hope to examine correlative and comparative methods that may improve the quality of data.

This is an introduction to session 29, Correlative Fluorescent Microscopy and Flow Cytometry Techniques, and the first time the Microscopy Society of America has sponsored a session that involves flow cytometry. Flow cytometry offers several statistical advantages over standard microscopies in evaluating populations of cells. Flow cytometry also facilitates a statistical precision in evaluating fluorescence and fluorescence changes in both chemical probes and transfected fluorescent proteins.

Type
Correlative Fluorescent Microscopy and Flow Cytometry Techniques (Organized by R. Smith)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 606 - 607
Copyright
Copyright © Microscopy Society of America 2001

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References

1.Smith, R. W., Proc. Ann. MSA Meeting, 59 (1999).Google Scholar
2.Shapiro, H. M., Practical Flow Cytometry, Third Ed., New YorkWiley-Liss (1995) 542 p.Google Scholar
3. This work possible through the generous encouragement of Dr. David T. Clark at Portland State University and the flow cytometry core facilities at Oregon Health Sciences University, the Oregon Cancer Center and the Veteran's Administration Medical Center, Portland, Oregon.Google Scholar