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An Interactive User Interface for Automated Acquisition of Transmission Electron Micrographs

Published online by Cambridge University Press:  02 July 2020

J. Pulokas ,
N. Kisseberth ,
C.S. Potter  and
B. Carragher
J. Pulokas
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
N. Kisseberth
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
C.S. Potter
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
B. Carragher
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
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Extract

For several years we have been developing a software application, called Leginon, for the automated control and acquisition of images from a transmission electron microscope. The system has been developed around a Philips CM200 and a Gatan MSC CCD camera. One of the primary motivations in developing this software is to provide for a system that can acquire many hundreds of images under low dose conditions from a specimen embedded in vitreous ice. In order to set up and manage this system we have developed a number of simple interactive graphical tools that enable the user to design, oversee and manage protocols for controlling the microscope and collecting the images. Many of these simple tools have also proved generally useful as stand alone applications.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 288 - 289
Copyright
Copyright © Microscopy Society of America

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References

1.Kisseberth, N., et al. (1998) JSB 120, 309–319.Google Scholar
2.Potter, C.S., et al. (1999) Ultramicroscopy, 77, 153–161.CrossRefGoogle Scholar
3.Pulokas, J., et al. (1999) JSB. 128, 250–256.Google Scholar
4.Ousterhout, J.K. (1994) Tel and the Tk Toolkit. Addison-Wesley.Google Scholar
5. Support is provided by the NSF (9730056 and 9904547) and the IBM Shared University Research program.Google Scholar