Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T19:50:28.046Z Has data issue: false hasContentIssue false
  • English
  • Français

All-Solid-State Diode-Pumped Fluorescence Lifetime Imaging System for Biomedicaine and Microscopy

Published online by Cambridge University Press:  02 July 2020

M. J. Cole ,
K. Dowling ,
P. M. W. French ,
R. Jones ,
D. Parsons-Karavassilis ,
M. J. Lever ,
A. K. L. Dymoke-Bradshaw ,
J. D. Hares ,
M. A. A. Neil  and
R. Ju_kaitis
...Show all authors
M. J. Cole
Affiliation:
Femtosecond Optics Group, Physics Dept, Imperial College, LondonSW7 2BZ, U.K., Tel: +44-171-594 7706, Fax: +44-171-594 7782, email: [email protected]
K. Dowling
Affiliation:
Femtosecond Optics Group, Physics Dept, Imperial College, LondonSW7 2BZ, U.K., Tel: +44-171-594 7706, Fax: +44-171-594 7782, email: [email protected]
P. M. W. French
Affiliation:
Femtosecond Optics Group, Physics Dept, Imperial College, LondonSW7 2BZ, U.K., Tel: +44-171-594 7706, Fax: +44-171-594 7782, email: [email protected]
R. Jones
Affiliation:
Femtosecond Optics Group, Physics Dept, Imperial College, LondonSW7 2BZ, U.K., Tel: +44-171-594 7706, Fax: +44-171-594 7782, email: [email protected]
D. Parsons-Karavassilis
Affiliation:
Femtosecond Optics Group, Physics Dept, Imperial College, LondonSW7 2BZ, U.K., Tel: +44-171-594 7706, Fax: +44-171-594 7782, email: [email protected]
M. J. Lever
Affiliation:
Department of Biological and Medical Systems, Imperial College, London, SW7 2BY, U.K.
A. K. L. Dymoke-Bradshaw
Affiliation:
Kentech Instruments Ltd., Unit 9, Hall Farm Workshops, South Moreton, Didcot, Oxon., OX11 9AG, U.K.
J. D. Hares
Affiliation:
Kentech Instruments Ltd., Unit 9, Hall Farm Workshops, South Moreton, Didcot, Oxon., OX11 9AG, U.K.
M. A. A. Neil
Affiliation:
Department of Engineering Science, University of Oxford, Parks Road, Oxford., OXI 3PJ, U.K.
R. Ju_kaitis
Affiliation:
Department of Engineering Science, University of Oxford, Parks Road, Oxford., OXI 3PJ, U.K.
T. Wilson
Affiliation:
Department of Engineering Science, University of Oxford, Parks Road, Oxford., OXI 3PJ, U.K.
Get access

Extract

The determination of fluorescence lifetime requires only relative measurements of intensity and so is especially useful for biomedical samples in which the heterogeneous nature of tissue and autofluorescence cause significant problems. Since fluorescence lifetime is dependent upon both radiative and non-radiative decay rates, it may be used to distinguish between different fluorophore molecules (with different radiative decay rates) and to monitor local environmental perturbations that affect the non-radiative decay rate. Fluorescence lifetime probes have been demonstrated for many biologically significant analytes including [O2], [Ca2+] and pH. Fluorescence lifetime imaging (FLIM) can be applied to almost any optical imaging modality, including microscopy and potentially to non-invasive optical biopsy. Fluorescence lifetime data may be acquired in the frequency or time domain. The recent development of user-friendly and relatively portable ultrafast laser technology and the availability of ultrafast gated optical image intensifiers (GOI’s) enable the development of potentially inexpensive time domain FLIM instruments that may be deployed outside specialist laser laboratories.

Type
Multi Photon Excitation Microscopy: The Next Generation
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1066 - 1067
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Lakowicz, J. R., “Principles of Fluorescence Spectroscopy”, Plenum Press (1983).CrossRefGoogle Scholar
2Szmancinski, H., et al., Methods Enzymol. 240 (1994) 723.CrossRefGoogle Scholar
3Cubeddu, R., et al. Opt. Eng. 32 (1993) 320.CrossRefGoogle Scholar
4Dowling, K, et al., Opt. Lett. 23 (1998) 810.CrossRefGoogle Scholar
5Neil, M. A. A., et al., Opt. Lett. 22 (1997) 1905.CrossRefGoogle Scholar
6Jones, R., et al., To be published in Electronics Letters 35 (4) (18 February, 1999).Google Scholar
7 This work has been supported by the UK Engineering and Physical Sciences Research Council and the Royal Society. MJC acknowledges a CASE studentship sponsored by the Institute of Cancer Research at the Royal Marsden NHS Hospital Trust. DP-K acknowledges an EPSRC studentship.Google Scholar