Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-02T19:07:56.034Z Has data issue: false hasContentIssue false

Accurately measuring ice thickness quickly and quantitatively on a screening TEM

Published online by Cambridge University Press:  30 July 2021

Hamish Brown
Affiliation:
The University of Melbourne, Parkville, Victoria, Australia
Eric Hanssen
Affiliation:
The University of Melbourne, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Cryo-EM at Local, Regional, and National Cryo-EM Centers
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Subramaniam, Sriram, et al. "Resolution advances in cryo-EM enable application to drug discovery." Current opinion in structural biology 41 (2016): 194-202.Google ScholarPubMed
Zivanov, Jasenko, et al. "New tools for automated high-resolution cryo-EM structure determination in RELION-3." elife 7 (2018): e42166.Google ScholarPubMed
Russo, Christopher J., and Passmore, Lori A.. "Ultrastable gold substrates for electron cryomicroscopy." Science 346.6215 (2014): 1377-1380.CrossRefGoogle ScholarPubMed
Baxter, William T., et al. "Determination of signal-to-noise ratios and spectral SNRs in cryo-EM low-dose imaging of molecules." Journal of structural biology 166.2 (2009): 126-132.Google ScholarPubMed
Noble, Alex J., et al. "Routine single particle CryoEM sample and grid characterization by tomography." Elife 7 (2018): e34257.CrossRefGoogle ScholarPubMed
Rice, William J., et al. "Routine determination of ice thickness for cryo-EM grids." Journal of structural biology 204.1 (2018): 38-44.CrossRefGoogle ScholarPubMed
Angert, Isabel, et al. "Elastic and inelastic scattering cross-sections of amorphous layers of carbon and vitrified ice." Ultramicroscopy 63.3-4 (1996): 181-192.CrossRefGoogle Scholar
We acknowledge use of the ThermoFischer Arctica and Tecnai F30 microscopes at the Ian Holmes Imaging centre, Bio21 Institute at the University of MelbourneGoogle Scholar