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Where Art Thou, Calcium?

Published online by Cambridge University Press:  02 July 2020

A.P. Somlyo
A.P. Somlyo*
Affiliation:
Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, Virginia
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Extract

Ever since the recognition of calcium as a major intracellular messenger of signal transduction, its subcellular localization and intracellular movements have been intensively sought through electron and light optical methods. Electron probe microanalysis (EPMA), X-ray mapping, electron energy-loss spectroscopy (EELS) and energy-filtered imaging still provide the highest spatial resolution for measuring total calcium, whereas with light optical methods (fluorescent, luminescent and absorbance dyes) free [Ca2+]i can be measured with high sensitivity and time resolution. This presentation will summarize the relationship, whether collision or convergence, between the results of electron and light optical methods, with particular reference to mitochondrial Ca, and consider the potential for further improvements in detection sensitivity and spatial resolution.

Sarcoplasmic and endoplasmic reticulum: Early attempts to quantitate Ca in cellular organelles with EPMA were directed at the sarcoplasmic reticulum (SR) of skeletal muscle, where EPMA could also address questions not amenable to studies of isolated SR.

Type
Quantitative Biological and Materials Microanalysis by Electrons and X-Rays
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 913 - 914
Copyright
Copyright © Microscopy Society of America 1997

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