Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-12-01T02:22:06.580Z Has data issue: false hasContentIssue false
  • English
  • Français

Visualization of the Transport Pathways of High Density Lipoproteins Across the Endothelial Cells in the Rat Arteries

Published online by Cambridge University Press:  02 July 2020

H. H. Lin ,
W. T. Chao  and
V. C. Yang
H. H. Lin
Affiliation:
Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China
W. T. Chao
Affiliation:
Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China
V. C. Yang
Affiliation:
Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China
Get access

Extract

An elevated low-density lipoprotein (LDL) in the plasma is the most recognized risk factor in the pathogenesis of atherosclerosis. On the other hand, the concentration of high density lipoprotein (HDL), is inversely correlated with atherosclerotic coronary artery disease. The transport pathways of LDL across the arterial wall has been well established. However, little is known about the transport mechanism(s) of HDL across the arterial wall. In this study, HDL was conjugated to colloidal gold for investigation of the ultrastructural aspects of binding and uptake of HDL by aortic endothelium.

High density lipoproteins were isolated from fresh rat plasma by sequential ultracentrifugation. HDL-colloidal gold conjugates were prepared according to the modified method of Kao et al. Sprague-Dawley rats were anesthetized and the vasculature was cleared of blood by perfusion with oxygenated PBS supplemented with 1 mM CaCl2 and 14 mM glucose at 37 °C through the abdominal aorta using the vena cava as an outlet.

Type
Cytochemistry (Light and Electron Histochemistry)
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1098 - 1099
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Wissler, R. W.. Am. J. Med. 91(suppl 1B) (1995) 1B.Google Scholar
2.Basil, M. and Rifkind, M. D.. Am. J. Cardiol. 66 (1990)3a.Google Scholar
3.Kao, C. H. et al., Atherosclerosis 116 (1995) 27.CrossRefGoogle Scholar