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Visualization Of The Transcription Factor Agl15 In Plant Embryos: A Case Of Regulation Via Cellular Localization

Published online by Cambridge University Press:  02 July 2020

D.E. Fernandez ,
S.E. Perry  and
M.D. Lehti
D.E. Fernandez
Affiliation:
Department of Botany, University of Wisconsin-Madison, Madison, WI53706
S.E. Perry
Affiliation:
Current address:Department of Agronomy, University of Kentucky, Lexington, KY40546
M.D. Lehti
Affiliation:
Department of Botany, University of Wisconsin-Madison, Madison, WI53706
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Extract

AGL15 (AGAMOUS-like 15) was originally identified as a sequence preferentially expressed in developing plant embryos by comparing PCR-generated ‘differential displays’ of embryo and non-embryo RNA populations. The sequence is expressed at low levels and encodes a member of the MADS domain family (named for the first four members to be identified: MCM1, AGAMOUS, DEFICIENS, and SERUM RESPONSE FACTOR) of regulatory factors. Members of the MADS domain family play pivotal roles in critical developmental events in a wide variety of organisms: in specification of mating type in yeast (MCM1), in responses to the environment in human cells (SRF), in control of mesoderm differentiation in Drosophila and muscle differentiation in humans (MEF2), and in determination of meristem and organ identity during flower development in plants (AGAMOUS, DEFICIENS/APETALA3, GLOBOSA/PISTILLATA, etc.). AGL15 is the only MADS domain factor identified to date that is expressed in a highly preferential manner in plant embryos.

Type
Application Of Correlative Microscopy To Studies Of Plant Cell Function
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1246 - 1247
Copyright
Copyright © Microscopy Society of America

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References

1.Heck, G.R. et al, Plant Cell 7(1995)1271.Google Scholar
2.Perry, S.E. et al., Plant Cell 8(1996)1977.Google Scholar
3.Perry, S.E. et al., Plant Physiol. (1999)in press.Google Scholar
4.This research was supported by grants from the NSF, USDA-NRICGP, DOE/NSF/USDA Collaborative Program in Plant Biology, and UW-Madison Graduate School.Google Scholar