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Video Light Microscopy

Published online by Cambridge University Press:  02 July 2020

K. R. Spring ,
B. Herman  and
E. D. Salmon
K. R. Spring
Affiliation:
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, 20892-0951
B. Herman
Affiliation:
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-7090
E. D. Salmon
Affiliation:
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3280
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Extract

This tutorial emphasizes the fundamentals of the use of light microscopy in combination with electronic imaging. The goal of the tutorial is to enable the attendees to make informed choices both about the modes of imaging best suited to their application and about the most suitable components to be employed. The didactic portion of the program will consist of five presentations, each dealing with different aspects of modern imaging systems and their applications to problems of biological significance. A laboratory demonstration session, organized to complement the lectures will follow.

The morning session will begin will a brief review by Dr. Spring of the principles of light detection and the formation of the video signal. Dr. Salmon will then describe video-enhanced contrast microscopy. This transmitted light imaging technique, widely utilized to visualize low contrast specimens exploits the wide dynamic range and high gain of video cameras for the capture of images that are virtually invisible through the eyepieces because of high background light.

Type
Tutorials
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 369 - 370
Copyright
Copyright © Microscopy Society of America 1997

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