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Ultrastructure of Septa from the Filamentous Fungus Aspergillus Nidulans

Published online by Cambridge University Press:  02 July 2020

Elizabeth A. Richardson  and
Michelle Momany
Elizabeth A. Richardson
Affiliation:
Department of Botany, University of Georgia, Athens, GA30602-7271USA
Michelle Momany
Affiliation:
Department of Botany, University of Georgia, Athens, GA30602-7271USA
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Extract

The filamentous fungus Aspergillus nidulans partitions its cells by laying down septa at regularly spaced intervals in response to nuclear division. Physiological and genetic studies of the temperature-sensitive sep mutants have been especially useful in dissecting the regulation of septation. Electron microscopic studies of the sep mutants should be equally useful in dissecting the structural intermediates of septation. In preparation for ultrastructural studies of the sep mutants, we have examined septa in wild-type A. nidulans fixed by freeze substitution.

Dialysis membranes were placed on rich medium plates and inoculated with A. nidulans spore suspensions. After 12 hours at 30°C, the dialysis membranes with adhering fungal hyphae were cut into square pieces measuring approximately 5mm on each side. The pieces were plunged into liquid propane and processed according to the procedures of Hoch. Serial sections were cut using a diamond knife and post stained with uranyl acetate and lead citrate.

Type
Biological Ultrastructure/Microbiology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1142 - 1143
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Harris, S. D. et al., Genetics (1994)136, 517.CrossRefGoogle Scholar
2.Momany, M. et al., Can. J. Bot. (1995)73, 396.CrossRefGoogle Scholar
3.Wolkow, T. D. et., J. Cell Sci. (1996)109, 2179.CrossRefGoogle Scholar
4.Hoch, H.C., in Ultrastructural Techniques for Microorganisms, Eds. Aldrich, H.C. and Todd, W.J., New York: Plenum (1986)183.CrossRefGoogle Scholar