Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-12-01T02:28:40.487Z Has data issue: false hasContentIssue false
  • English
  • Français

Ultrastructural Distribution of Basic Fibroblast Growth Factor-Like Molecules in Peripheral Nerves

Published online by Cambridge University Press:  02 July 2020

Robert J. Kayton  and
Ranan Gulhan Aktas
Robert J. Kayton
Affiliation:
Oregon Health Sciences University, C.R.O.E.T., L606, Portland, OR97001
Ranan Gulhan Aktas
Affiliation:
Trakya University, Faculty of Medicine, Histology and Embryology Department, Edirne/Turkey
Get access

Extract

Basic Fibroblast Growth Factor (bFGF) is a multifunctional polypeptide which has been shown to play a pivotal role in the survival and differentiation of nerve cells. Several trophic and non-trophic functions of this protein have been suggested in peripheral nerves. In spite of ample information about the distribution and effects of bFGF in central nervous system, few data are available concerning the localization of this protein in peripheral nerves. In view of the role of bFGF in regulation of trophic and non-trophic functions, we particularly focused on the presence and precise location of bFGF in peripheral nerves at the electron microscope level.

Spurr's resin embedded ultrathin sections from adult rats’ sural nerves were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-Zymogenetics) specific for bFGF using two-step immunogold labeling method. Control samples were treated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, VGF, anti-histamine, anti-fibroblast 5B5).

Type
Cytochemistry (Light and Electron Histochemistry)
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1102 - 1103
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1-Baird, A., Curr. Op. Neurobiol. 4(1994)78.CrossRefGoogle Scholar
2-Grothe, C. et al., Neuroscience 76(1997)123.CrossRefGoogle Scholar
3-Otto, D. et al., Neuroscience Letters 83(1987)156.CrossRefGoogle Scholar
4-Unsicker, K. et al., Proc. Natl. Acad. Sci.USA 84(1987)5459.CrossRefGoogle Scholar
5-Wagner, J. A., Curr. Top. Microb. Immun. 165(1991)95.Google Scholar
6-Morgan, L. et al., Development 120(1994)1399.CrossRefGoogle Scholar
7-Hassan, S. M. et al., Acta Neuropathol. 87(1994)405.CrossRefGoogle Scholar
8-Gospodarowicz, D. et al., Endocr. Rev. 8(1987)95.CrossRefGoogle Scholar
9-Davis, J. B. et al., J. Cell Biol. 110(1990)1353.CrossRefGoogle Scholar
10-Schubert, D. et al., J. Cell Biol. 104(1987)635.CrossRefGoogle Scholar