Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-28T13:21:37.175Z Has data issue: false hasContentIssue false
  • English
  • Français

Three-Dimensional Structure of Isometric Capsids of Bacteriophage T4

Published online by Cambridge University Press:  02 July 2020

T. S. Baker ,
N. H. Olson ,
M. Gingery  and
F. A. Eiserling
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
N. H. Olson
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
M. Gingery
Affiliation:
Department of Microbiology & Molecular Genetics, University of California, Los Angeles, CA90095-1489
F. A. Eiserling
Affiliation:
Department of Microbiology & Molecular Genetics, University of California, Los Angeles, CA90095-1489
Get access

Extract

Coliphage T4 is a large, double-stranded DNA virus (family Myoviridae) which infects Escherichia coli. It is the classic example of a contractile-tailed phage and a wealth of information on viral morphogenesis processes have been obtained on it. Extensive studies of T4 by negative-stain and metal-shadow electron microscopy have shown that mature virions, which contain over 40 different polypeptides, have a prolate capsid that encapsidates the genome (∼175 × 106Da), and several other structural components, including the collar with whiskers, neck, a contractile sheath, and a baseplate with fibers. A complete understanding of processes such as assembly of the viral capsids will require atomic-scale knowledge of the various protein interactions. Details of the process can be extrapolated from knowledge of the initial, transitional, and final states of a structure.

We have initiated an examination of T4 head morphogenesis by studying mutant, isometric capsids ('isometrics’) using cryo-electron microscopy and three-dimensional (3D) image reconstruction methods as applied to particles with icosahedral symmetry.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 254 - 255
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Eiserling, F. A., and Black, L. W., Eds., in Karam, J., Ed., Molecular Biology of Bacteriophage T4. American Society for Microbiology, Washington, D.C. (1994)207.Google Scholar
2.Baker, T. S., et al., Microbio. Mol. Biol. Rev. 63(1999)862.CrossRefGoogle Scholar
3.Branton, D. and Klug, A.. J. Mol. Biol. 92(1975)559.CrossRefGoogle Scholar
4.Branton, D. and Klug, A.. J. Mol. Biol. 98(1975)445.CrossRefGoogle Scholar
5.Caspar, D.L.D. and Klug, A.. Cold Spring Harb. Symp. Quant. Biol. 27(1962)1.CrossRefGoogle Scholar
6.Aebi, U., et al. J. Mol. Biol. 110(1977)687.CrossRefGoogle Scholar
7.Yanagida., M.J. Mol. Biol. 109(1977)515.CrossRefGoogle Scholar
8.Müller-Salamin, L., et al., J. Virol. 24(1977)121.CrossRefGoogle Scholar
9. We thank R. Ashmore for programming. Research supported in part by a grant from the NIH (GM33050) and a shared instrumentation grant from NSF (BIR 9112921) to T.S.B., and research funds from the University of California to F.A.E.Google Scholar