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A Technique for In Vivo Morphological Evaluation of Chronically Implanted Neuronal Silicon Substrate Electrodes for Confocal Microscopy

Published online by Cambridge University Press:  02 July 2020

C.A. Edwards ,
P.A. Finger ,
D.J. Anderson ,
J.A. Wiler ,
J.F. Hetke  and
R.A. Altschuler
C.A. Edwards
Affiliation:
Department of Anatomy and Cell Biology, University of Michigan, 48109.
P.A. Finger
Affiliation:
Kresge Hearing Research Institute, University of Michigan, 48109.
D.J. Anderson
Affiliation:
Department of Engineering and Computer Science, University of Michigan, 48109.
J.A. Wiler
Affiliation:
Kresge Hearing Research Institute, University of Michigan, 48109.
J.F. Hetke
Affiliation:
Department of Engineering and Computer Science, University of Michigan, 48109.
R.A. Altschuler
Affiliation:
Kresge Hearing Research Institute, University of Michigan, 48109.
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Extract

Various multichannel silicon electrodes have been developed for stimulating and recording in the central nervous system of experimental animals. These electrodes can be used in their planar form or can be assembled into 3-D structures for volume interaction with tissue. However, bio-compatibility issues arise concerning the introduction of any electrode into living tissue. From earlier observations, we noticed that the neuronal cells had a tendency to adhere to the chronically implanted silicon substrate. Routine microtechnique became an obstacle when attempting to section the implanted and embedded electrode. Most often, this was attempted by passing a stainless steel blade through paraffin, or plastic embedded tissue, resulting in disrupting the tissue material interface. We needed to develop a reproducible procedure which preserved the integrity of this interface for future LM and confocal microscopic studies.

In order to preserve the electrode/tissue interface, we used the Exakt microgrinding/micropolishing technique, where the electrode/brain block was embedded in Technovit 7200 resin and cut with a diamond impregnated band saw.

Type
Technologists’ Forum: Special Topics and Symposium
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 351 - 352
Copyright
Copyright © Microscopy Society of America 1997

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References

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