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The Study Of Meiosis-I In Maize By Confocal Microscopy And Digital Deconvolution

Published online by Cambridge University Press:  02 July 2020

Thomas C. Lee ,
David B. Walden  and
Ping-chin Cheng
Thomas C. Lee
Affiliation:
Williamsville North HS, , Williamsville, NY14221, USA
David B. Walden
Affiliation:
Dept. of Plant Sciences, Univ. of Western Ontario, London, Ontario, Canada, N6A 5B7
Ping-chin Cheng
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical Engineering, State Univ. of New York, Buffalo, NY14260, USA
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Extract

Visualization is interdependent with gene mapping and DNA sequencing, because spatial relationships are important to understand function. The ability to visualize the chromosomes, especially in threedimensional (3D) space, has great benefits for the better understanding of a gene's function. For these reasons, a better technique is needed to study the chromosomes in 3D. Studying the stages of meiosis I provides an excellent opportunity to evaluate the capability of 3D imaging techniques. The loosening of the chromosomes in meiosis I exemplify the advantages of 3D imaging.

Tassel inflorescence from maize (Zea mays L., inbreed Ohio43) was collected from the field station at the University of Western Ontario in London, Ontario, Canada during the summer of 1998. Tassels at proper developmental stages were fixed in 3:1 (EtOH / acetic acid) solution. The anthers were stained with the Feulgen method and cleared in methyl salicylate.

Type
Ultrastructural Analysis Of Plant Cells
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1264 - 1265
Copyright
Copyright © Microscopy Society of America

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References

References:

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