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Overexpression of MicroRNA-29b Decreases Expression of DNA Methyltransferases and Improves Quality of the Blastocysts Derived from Somatic Cell Nuclear Transfer in Cattle

Published online by Cambridge University Press:  27 February 2018

Shuang Liang ,
Zheng-Wen Nie ,
Jing Guo ,
Ying-Jie Niu ,
Kyung-Tae Shin ,
Sun A. Ock  and
Xiang-Shun Cui
Shuang Liang
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea Department of Animal Sciences, College of Animal Sciences, Jilin University, Changchun, 130062, China
Zheng-Wen Nie
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea
Jing Guo
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Insititute of Chinese Academy of Agricultural Sciences, Harbin 150069, China
Ying-Jie Niu
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea
Kyung-Tae Shin
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea
Sun A. Ock
Affiliation:
Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeonju 55365, Republic of Korea
Xiang-Shun Cui*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea
*
Author for correspondence: Xiang-Shun Cui, E-mail: [email protected]
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Abstract

MicroRNA (miR)-29b plays a crucial role during somatic cell reprogramming. The aim of the current study was to explore the effects of miR-29b on the developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos, as well as the underlying mechanisms of action. The expression level of miR-29b was lower in bovine SCNT embryos at the pronuclear, 8-cell, and blastocyst stages compared with in vitro fertilized embryos. In addition, miR-29b regulates the expression of DNA methyltransferases (Dnmt3a/3b and Dnmt1) in bovine SCNT embryos. We further investigated SCNT embryo developmental competence and found that miR-29b overexpression during bovine SCNT embryonic development does not improve developmental potency and downregulation inhibits developmental potency. Nevertheless, the quality of bovine SCNT embryos at the blastocyst stage improved significantly. The expression of pluripotency factors and cellular proliferation were significantly higher in blastocysts from the miR-29b overexpression group than the control and downregulation groups. In addition, outgrowth potential in blastocysts after miR-29b overexpression was also significantly greater in the miR-29b overexpression group than in the control and downregulation groups. Taken together, these results demonstrated that miR-29b plays an important role in bovine SCNT embryo development.

Keywords

MicroRNA-29bbovinesomatic cell nuclear transfer (SCNT)embryo development
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 24 , Issue 1 , February 2018 , pp. 29 - 37
Copyright
© Microscopy Society of America 2018 

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Footnotes

a

Shuang Liang and Zheng-Wen Nie equally contributed to this work.

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