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Muscle Biopsy: Use of Cryosections, Histochemistry, and Electron Microscopy for Diagnosis

Published online by Cambridge University Press:  02 July 2020

Cindy L. Hastings ,
Yvonne M. Jones  and
Robert E. Mrak
Cindy L. Hastings
Affiliation:
Department of Veterans’ Affairs Medical Center, Little Rock, Arkansas, 72205.
Yvonne M. Jones
Affiliation:
Department of Veterans’ Affairs Medical Center, Little Rock, Arkansas, 72205.
Robert E. Mrak
Affiliation:
Department of Veterans’ Affairs Medical Center, Little Rock, Arkansas, 72205.
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Extract

Modern diagnosis of biopsied muscle requires a multidisciplinary approach, including routine histology and immunohistochemistry, cryosectioning for histochemistry, and (in selected cases) electron microscopy or biochemical evaluation. Routine paraffin embedding offers excellent histological detail. Cryosections provide fresh tissue for histochemical reactions that delineate fiber types and reveal special architectural alterations. Electron microscopy is applied in selected instances to provide diagnostic detail not otherwise apparent. Biochemical analysis of frozen tissue is used to define certain inborn errors of metabolism. This integrated approach allows diagnostic precision far beyond the limited information available from routine paraffin sections. We present examples of diagnoses made possible using these special techniques.

Chronic, slowly progressive neuropathies show grouping of fiber types and target fiber formation, apparent in special histochemical preparations. Routine histological preparations may be normal or show only nonspecific changes. Central core disease shows areas of pallor evident in the central areas of many myofibers in histochemical preparations.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1082 - 1083
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Mrak, RE, Lange, B, Brodsky, MC: Broad A-bands of striated muscle in Leber's congenital amaurosis. A new congenital myopathy? Neurology 43:838–841, 1993.CrossRefGoogle ScholarPubMed
2.Mrak, RE, Griebel, M, Brodsky, MC: Broad A band disease: a new, benign congenital myopathy. Muscle Nerve 19:587–594, 1996.3.0.CO;2-7>CrossRefGoogle Scholar
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