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Mouse Sperm Association During Epididymal Transit

Published online by Cambridge University Press:  02 July 2020

M. Monclus ,
M. H. Burgos  and
M.W. Fornés
M. Monclus
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
M. H. Burgos
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
M.W. Fornés
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
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Extract

Head-to-head sperm association was described in some species as a normal phenomenon during epididyma transit (Phillips and Bedford, 1987). Recently, a new sperm head association (SHA) was characterized in the rat (Fornés & Burgos 1990; 1994).

At this moment, the study of SHA was extended to mouse sperm. SHA is a type of cell-cell interaction clearly observed when an a drop of epididymal fluid, obtained by a puncture of epididymal duct, is placed in a culture medium (HMB media: Modified Ringers medium).

This preparation can be observed under contrast phase microscope (optiphot-2, Nikkon) with or without fixative (2% paraformaldehyde and 2% glutaraldehyde in 0.01 M PBS buffer, pH 7,2). The mass of spermatozoa begin to move but not as a single sperm. They move as a clump of sperm and without fixative the sperm motility alone disaggregate the SHA.

Type
Developmental/Reproductive Biology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 968 - 969
Copyright
Copyright © Microscopy Society of America

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References

References:

Phillips, D. M. and Bedford, J.M.. Gamete Res. 18 (1988): 17–25.CrossRefGoogle Scholar
Fornes, M. W. y Burgos, M. H.. Mia. Electr. Biol. Cel. 14 (2) (1990): 115–130.Google Scholar
Fornes, M.W. y Burgos, M.H.. Mol. Reprod. Develop.: 38 (1994): 43–47.CrossRefGoogle Scholar