Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-26T07:11:29.201Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphometric Analysis Of Polychlorinated Biphenyl 118-Induced Ultrastructural Alterations In The Rat Liver

Published online by Cambridge University Press:  02 July 2020

T.L. Gallant  and
A. Singh
T.L. Gallant
Affiliation:
Department of Biology, University of Prince Edward Island., Charlottetown, PE, CanadaCIA 4P3
A. Singh
Affiliation:
Atlantic Veterinary College., Charlottetown, PE, CanadaCIA 4P3
Get access

Extract

Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants that bioaccumulate in the food chain and thus pose a health risk to human beings and other animals. The exceptional dielectric and lubricating properties of the PCBs resulted in their extensive use during the mid-1950s as lubricants in electrical generators and transformers. PCBs have a long half-life and persist in the environment causing concerns related to their toxicity. The present research on congener 118 (2,3',4,4',5-pentachlorobiphenyl) involves the quantification of hepatocyte alterations using stereology techniques. Application of a quantitative technique to estimate liver pathology confers more confidence in evaluating toxicity of a xenobiotic, and in the determination of its NOAEL (No Observable Adverse Effect Level).

Alterations in the volume fraction of cellular constituents in the hepatocytes induced by congener 118 were estimated relative to the sex of, and dose fed to a laboratory population of weanling Sprague-Dawley rats. The rats were given the PCB dose in concentrations of 2, 200, and 2,000 ppb to females and 10, 1,000 and 10,000 ppb to males; three animals were used in each dose group.

Type
Biological Ultrastructure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1266 - 1267
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Kimbrough, R. D., Critical Rev. Toxicol. 25(1995)133.CrossRefGoogle Scholar
2.Peng, J. et al., Toxicology 120(1997)171.CrossRefGoogle Scholar
3.MacLellan, K. et al., J. Submicrosc. Cytol. Pathol. 26(1994)279.Google Scholar
4. The Natural Sciences & Engineering Research Council of Canada is acknowledged for financial support. Appreciation is extended to Mr. Barry Connell, who assisted in laboratory work,and Mr. Ameet Singh for aid in literature searches for the PCB comprehensive study.Google Scholar