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Morphological Changes in the Myotendinous Junction of mdx Mice

Part of: Micrographia Collection

Published online by Cambridge University Press:  11 August 2021

Giovana Zerbo Martinez ,
Bruna Aléxia Cristofoletti Grillo Open the ORCID record for Bruna Aléxia Cristofoletti Grillo [Opens in a new window] ,
Lara Caetano Rocha Open the ORCID record for Lara Caetano Rocha [Opens in a new window] ,
Carolina dos Santos Jacob Open the ORCID record for Carolina dos Santos Jacob [Opens in a new window] ,
Jurandyr Pimentel Neto Open the ORCID record for Jurandyr Pimentel Neto [Opens in a new window] ,
André Neri Tomiate Open the ORCID record for André Neri Tomiate [Opens in a new window] ,
Gabriela Klein Barbosa Open the ORCID record for Gabriela Klein Barbosa [Opens in a new window] ,
Ii-sei Watanabe Open the ORCID record for Ii-sei Watanabe [Opens in a new window]  and
Adriano Polican Ciena Open the ORCID record for Adriano Polican Ciena [Opens in a new window]
Giovana Zerbo Martinez
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Bruna Aléxia Cristofoletti Grillo
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Lara Caetano Rocha
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Carolina dos Santos Jacob
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Jurandyr Pimentel Neto
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
André Neri Tomiate
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Gabriela Klein Barbosa
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Ii-sei Watanabe
Affiliation:
Department of Anatomy, Institute of Biomedical Sciences III, University of São Paulo (USP), São Paulo, SP, Brazil
Adriano Polican Ciena*
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
*
*Correspondent author: Adriano Polican Ciena, E-mail: [email protected]
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Abstract

The myotendinous junction (MTJ) is the interface between muscle and tendon, and it is the main area of force transmission of the locomotor apparatus. Dystrophic processes promote pathological injury which affects the skeletal muscle and can influence the morphology of the MTJ. This study aimed to investigate the adaptations in MTJ morphology of mdx mice in the tibialis anterior muscle. Male mice (n = 24) were divided into Control—C57bl/10 and mdx—C57bl/10mdx (Duchenne muscular dystrophy experimental model). In the mdx group, centralized nuclei with a large area and greater deposition of type III collagen (fibrosis) were observed. Also, shorter sarcomeres and sarcoplasmatic projections of MTJ were observed. We concluded that the adaptations in mdx mice demonstrated extensive impairment in the MTJ region with reduced ultrastructures.

Keywords

Duchenne muscular dystrophyfibrosisfinger-like processmuscle degenerationsarcomere
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1290 - 1294
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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