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Localization of Z-RNA in Normal Lens Epithelium: Middle Fibers

Published online by Cambridge University Press:  02 July 2020

C.E. Gagna ,
H.R. Kuo ,
R. Schulz ,
R. Cordova ,
G. Crippen  and
W.C. Lambert
C.E. Gagna
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA Department of Life Sciences, NYIT, Old Westury, NY11508USA department of Biology, FDU, Teaneck, NJ07666USA
H.R. Kuo
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA
R. Schulz
Affiliation:
department of Biology, FDU, Teaneck, NJ07666USA
R. Cordova
Affiliation:
Department of Life Sciences, NYIT, Old Westury, NY11508USA
G. Crippen
Affiliation:
Department of Life Sciences, NYIT, Old Westury, NY11508USA
W.C. Lambert
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA
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Extract

The goal of this project was to analyze the cellular localization of Z-RNA, within middle fibers (MF) of the adult dog ocular lens (1.5 yr) (Fig. 1), using anti-Z-RNA IgG polyclonal antibodies. B-DNA can adopt the left-handed Z-DNA conformation in vitro (1). Right-handed A-RNA can be transformed into left-handed Z-RNA (2). Z-RNA has been studied in cultured cells (3). Evidence supports the presence of Z-DNA in vivo (1). Removal of DNA binding proteins by fixatives can initiate supercoiling which stabilizes Z-DNA sequences (1).

Anti-Z-RNA polyclonal antibody probes were developed in rabbits immunized with multiple injections of Z-RNA: Br-poly[ribosomal(G-C)]. Regarding immunohistochemistry, lens tissues were fixed in Carnoy's, embedded in paraffin and sectioned (2.5 μm) (Fig. 2). Computerized image analysis was performed using a Leitz DM-RB microscope and Leica Quantiment 500 + image analyzer.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 900 - 901
Copyright
Copyright © Microscopy Society of America

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References

References:

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