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Localization of the Trace Elements Iron, Zinc and Selenium in Relation to Anatomical Structures in Bovine Ovaries by X-Ray Fluorescence Imaging

Published online by Cambridge University Press:  08 April 2015

Melanie J. Ceko ,
Katja Hummitzsch ,
Wendy M. Bonner ,
Jade B. Aitken ,
Kathryn M. Spiers ,
Raymond J. Rodgers  and
Hugh H. Harris
Melanie J. Ceko
Affiliation:
Department of Chemistry, The University of Adelaide, SA 5005, Australia
Katja Hummitzsch
Affiliation:
Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Research Institute, The University of Adelaide, SA 5005, Australia
Wendy M. Bonner
Affiliation:
Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Research Institute, The University of Adelaide, SA 5005, Australia
Jade B. Aitken
Affiliation:
School of Chemistry, The University of Sydney, NSW 2006, Australia
Kathryn M. Spiers
Affiliation:
Australian Synchrotron, Clayton, VIC 3168, Australia
Raymond J. Rodgers
Affiliation:
Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Research Institute, The University of Adelaide, SA 5005, Australia
Hugh H. Harris*
Affiliation:
Department of Chemistry, The University of Adelaide, SA 5005, Australia
*
*Corresponding author. [email protected]
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Abstract

X-ray fluorescence (XRF) was used to image 40 histological cross-sections of bovine ovaries (n=19), focusing on structures including: antral follicles at different stages of growth or atresia, corpora lutea at three stages of development (II–IV), and capillaries, arterioles, and other blood vessels. This method identified three key trace elements [iron (Fe), zinc (Zn), and selenium (Se)] within the ovarian tissue which appeared to be localized to specific structures. Owing to minimal preprocessing of the ovaries, important high-resolution information regarding the spatial distribution of these elements was obtained with elemental trends and colocalizations of Fe and Zn apparent, as well as the infrequent appearance of Se surrounding the antrum of large follicles, as previously reported. The ability to use synchrotron radiation to measure trace element distributions in bovine ovaries at such high resolution and over such large areas could have a significant impact on understanding the mechanisms of ovarian development. This research is intended to form a baseline study of healthy ovaries which can later be extended to disease states, thereby improving our current understanding of infertility and endocrine diseases involving the ovary.

Keywords

X-ray fluorescencebovine ovariesironzincselenium
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 3 , June 2015 , pp. 695 - 705
Copyright
© Microscopy Society of America 2015 

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