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Imaging Cell Surface Macromolecular Distribution by Mapping Intermolecular Interactions with an Atomic Force Microscope

Published online by Cambridge University Press:  02 July 2020

R. Bhatia ,
H. Lin ,
A. Quist ,
G. Primbs ,
N. Desai  and
R. Lai
R. Bhatia
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
H. Lin
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
A. Quist
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
G. Primbs
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
N. Desai
Affiliation:
NutraSweet Company, Mount Prospect, IL60056.
R. Lai
Affiliation:
Neuroscience Research Institute, University of California, Santa Barbara, CA93106
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Extract

An atomic force microscope (AFM) can image real-time intermolecular interactions between complementary macromolecules, such as receptor-ligand and antigen-antibody with nanometer resolution in hydrated state. Recent developments in AFM imaging allow mapping of such interaction forces over a large surface area such that local as well as global distribution of complementary biomolecules could be ascertained simultaneously. Using the force-volume maps and AFM probe conjugated with antibody, we have mapped the reorganization of specific receptors on the cell surface as well as the resultant changes in cellular micromechanical properties, such as elasticity and cytoskeletal reorganization of the cell (Figure 1).

In present study, we have mapped vascular endothelial growth factor receptor (VEGF-R) in the plasma membrane of cultured endothelial cells using anti-VEGFR - antibody conjugated to AFM probe. VEGF induced changes in cytoskeleton reorganization in endothelial cells were observed by real-time AFM imaging.

Type
Biomaterials
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 976 - 977
Copyright
Copyright © Microscopy Society of America

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