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Identification of Left-Handed RNA in the Cells of the Meridional Rows of the Normal Adult Mammalian Ocular Lens

Published online by Cambridge University Press:  02 July 2020

C.E. Gagna ,
H.-R. Kuo ,
J. Turley ,
J. Spencer ,
N. Rescinti  and
W.C. Lambert
C.E. Gagna
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ, 07103-2714, USA
H.-R. Kuo
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ, 07103-2714, USA
J. Turley
Affiliation:
School of Allied Health & Life Sciences, NYIT, Old Westbury, NY, 11568-8000, USA
J. Spencer
Affiliation:
School of Allied Health & Life Sciences, NYIT, Old Westbury, NY, 11568-8000, USA
N. Rescinti
Affiliation:
School of Natural Sciences, FDU, Teaneck, NJ, 07666-1914, USA
W.C. Lambert
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ, 07103-2714, USA
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Abstract

The purpose of this research project was to characterize the distribution of left-handed Z-RNA sequences within the epithelial cells of the adult noncataractous crystalline dog lens: the meridional rows (MR). This was achieved by using anti-Z-RNA IgG polyclonal antibody probes. Both light microscopy (LM) and electron microscopy (EM) were used to analyze the tissue binding of the anti- Z-RNA antibodies. Nucleic acids can adopt many different helical conformations (1), such as Z-DNA (Fig. 1) and Z-RNA (2,3). The lens is made up of a single cell type, which is a monolayer of undifferentiated epithelial cells covering its anterior surface (Fig. 2). At the equator of the lens, these cells elongate and form concentric layers of the secondary (nucleated) fiber cells, which undergo cell death-terminal differentiation (denucleation). The epithelial monolayer consists of several cell types, each of which has unique characteristics.

The production of anti-Z-RNA polyclonal antibody probes was achieved using four

Type
Labeling for Microscopy and Correlative Microscopy (Organized By R. Albrecht)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 1042 - 1043
Copyright
Copyright © Microscopy Society of America 2001

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References

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