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From Dynamics to Details: Live-Cell Light Microscopy and Highresolution (25 nm) Soft X-Ray Microscopy

Published online by Cambridge University Press:  02 July 2020

C. A. Larabell ,
D. Yager ,
W. Meyer-Ilse  and
B. A. Rowning
C. A. Larabell
Affiliation:
Life Sciences Division and Center for X-ray Optics, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA94720
D. Yager
Affiliation:
Life Sciences Division and Center for X-ray Optics, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA94720
W. Meyer-Ilse
Affiliation:
Center for X-ray Optics, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA94720
B. A. Rowning
Affiliation:
Life Sciences Division and Center for X-ray Optics, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA94720
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Extract

Imaging cells using a variety of microscopy techniques has generated information about the organization of cells and subcellular structures that is the foundation for understanding cell function. Invaluable information about the dynamics of cells has been obtained from a variety of light microscopy techniques, and the exquisite structural details of cellular organization have been obtained from electron microscopy. Each of these approaches, however, has its limitations. The resolution provided by light microscopy is limited by the wavelength of light, whereas the specimen preparation required for examining cells using electron microscopy is extremely tedious and time consuming. We show that soft x-ray microscopy can bridge the gap between these two forms of microscopy by providing a method for examining whole, hydrated cells up to 10 um thick at 25 nm resolution. Examination of rapidly frozen cells provides information that closely approximates that seen in living cells.

Type
Philadelphia—The Other Motor City: Muscle and Non-Muscle Motility. A Dedication to Dr. Lee Peachey
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 84 - 85
Copyright
Copyright © Microscopy Society of America

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