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Force Modulation Elasticity Mapping of Plastic-embedded, Thin-sectioned Skeletal Muscle

Published online by Cambridge University Press:  02 February 2002

Boris B. Akhremitchev ,
Henry G. Brown ,
Scott R. Graner  and
Gilbert C. Walker
Boris B. Akhremitchev
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260
Henry G. Brown
Affiliation:
Department of Pathology and Laboratory Medicine, Allegheny General Hospital, 320 East North Avenue, Pittsburgh, PA 15212
Scott R. Graner
Affiliation:
Department of Pathology and Laboratory Medicine, Allegheny General Hospital, 320 East North Avenue, Pittsburgh, PA 15212
Gilbert C. Walker*
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260
*
*Corresponding author
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Abstract

We have been researching the capability of atomic force microscopy to reveal nontopographic properties of tissue embedded in plastic and sectioned with standard electron microscopic techniques. We present topography and elasticity maps of plastic-embedded, thin sections of muscle tissue. The images show topography correlated with the normal repeating structure of the sarcomere. Elasticity mapping using force modulation revealed contrast between the actin- and myosin-rich areas. We attribute the observed contrast in elasticity to the difference in local concentrations of biological material in embedding plastic.

Keywords

atomic force microscopyscanning force microscopyforce-modulationelasticity mappingmechanical propertiesmuscleatomic force microscopyscanning force microscopytissue
Type
Research Article
Information
Microscopy and Microanalysis , Volume 7 , Issue 1 , January 2001 , pp. 32 - 38
Copyright
Copyright © Microscopy Society of America 2001

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