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Fish Erythrocytes as Biomarkers for the Toxicity of Sublethal Doses of an Azo Dye, Basic Violet-1 (CI: 42535)

Published online by Cambridge University Press:  01 December 2014

Kirandeep Kaur  and
Arvinder Kaur
Kirandeep Kaur
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, India
Arvinder Kaur*
Affiliation:
Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, India
*
*Corresponding author. [email protected]
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Abstract

The aim of the present study was to investigate poikilocytosis in Labeo rohita (an important food fish) as an early indicator of stress due to an azo dye, Basic Violet-1 (CI: 42535). This dye was observed to be very toxic to test fish (96 h LC50 as0.45 mg/L dye). Fish were given short-term (96 h) and subchronic (150 days) exposures to the dye, and poikilocytosis was recorded under light and scanning electron microscopy (SEM). Light microscopy helped in identification of micronuclei along with irregularities, notches, blebs, lobes, crenation, clumps, chains, spherocytes, vacuolation, and necrosis in erythrocytes. However, SEM indicated shrinkage, oozing of cytoplasm, and several new abnormal shapes including marginal foldings, discocytes, keratocytes, dacrocytes, degmacytes, acanthocytes, echinocytes, protuberances, stomatocytes, drepanocytes, holes in the membrane, stippling/spicules, crescent-shaped cells, triangular cells, and pentagonal cells. Earlier studies speculated changes in the membrane to be responsible for clumping and chaining of erythrocytes, whereas the present SEM study clearly indicates that oozing out of cytoplasm is also responsible for the formation of chains and clumps. This study also shows that erythrocytes exhibit pathological symptoms before the appearance of other external symptoms such as abnormal behavior or mortality of fish. There was a dose- and duration-dependent increase; therefore, poikilocytosis, especially echinocytes, spherocytes, and clumps, can act as a biomarker for the stress caused by azo dyes.

Keywords

poikilocytosisBasic Violet-1scanning electron microscopybiomarkerLabeo rohita
Type
Biological and Biomaterials Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 1 , February 2015 , pp. 264 - 273
Copyright
© Microscopy Society of America 2014 

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References

Adeyemo, O.K. (2007). Hematological profile of Clarias gariepinus (Burchell, 1822) exposed to lead. Turk J Fish Aquat Sc 7, 163–169.Google Scholar
Al-Sabti, K. (1986). Comparative micronucleated erythrocyte cell induction in three cyprinids by five carcinogenic-mutagenic chemicals. Cytobios 47, 147–154.Google Scholar
Amin, K.A., Hameid, H.A. II & Abd Elsttar, A.H. (2010). Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem Toxicol 48, 2994–2999.Google Scholar
Antonio, F.F., Jorge, V.F.C., Sofia, G.S., Sandra, M.M., Joao, C., Pedro, M. & Antanio, F.F. (2007). Histopathological changes in liver and gill epithelium of Nile tilapia Oreochromis niloticus, exposed to waterborne copper. Pesq Vet Bras 27, 103–109.Google Scholar
APHA, AWWA & WEF (2005). Standard Methods for the Examination of Water and Wastewater, 21st ed. Washington, DC: American Publication of Health Association.Google Scholar
Bagdonas, E. & Lazutka, R.J. (2007). Evaluation of DNA damage by means of comet assay and micronucleus test in erythrocytes of Prussian carp (Carassius auratus gibelio) infected with ulcerative disease. Biologija 53, 1–5.Google Scholar
Bailey, G.S., Williams, D.E. & Hendricks, J.D. (1996). Fish models for environmental carcinogenesis, the rainbow trout. Environ Health Perspect 104, 5–21.Google Scholar
Bansal, A.K. (2005). Modulation of N-nitrosodiethylamine induced oxidative stress by vitamin E in rat erythrocytes. Human Exp Toxicol 24, 297–302.Google Scholar
Basak, P.K. & Konar, S.K. (1977). Estimation of safe concentration of insecticide, a new method tested on DDT and BHC. J Inland Fish Soc India 9, 19–29.Google Scholar
Beauvais, S.L., Jones, S.B., Parris, J.T., Brewer, S.K. & Edward, E. (2001). Little cholinergic and behavioral neurotoxicity of carbaryl and cadmium to larval rainbow trout (Oncorhynchus mykiss). Ecotoxicol Environ Saf 49, 84–90.Google Scholar
Bester, J., Buys, A.V., Lipinski, B., Kell, D.B. & Pretorius, E. (2013). High ferritin levels have major effects on the morphology of erythrocytes in Alzheimer’s disease. Front Aging Neurosci 5, 88.Google Scholar
Carmen, Z. & Daniela, S. (2012). Textile organic dyes – Characteristics, polluting effects and separation/elimination procedures from industrial effluents – A critical overview. In Organic Pollutants Ten Years After the Stockholm Convention – Environmental and Analytical Update, Puzyn, T. (Ed.), pp. 55–86. Europe: InTech (ISBN: 978-953-307-917-2).Google Scholar
Chang, J.S., Chou, C., Lin, Y., Ho, J. & Hu, T.L. (2001). Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola . Water Res 35, 2041–2850.Google Scholar
Chequer, F.M.D., Angeli, J.P.F., Ferraz, E.R.A., Tsuboy, M.S., Marcarini, J.C., Mantovani, M.S. & Oliveira, D.O. (2009). The azo dyes disperse red 1 and disperse orange 1 increase the micronuclei frequencies in human lymphocytes and in HepG2 cells. Mutat Res Gen Tox En 676, 83–86.Google Scholar
Das, M., Ghosh, N., Chattopadhyay, D., Addya, S. & Chaterjee, G.C. (1988). Effects of acute oral administration of cadmium chloride on uptake of element and control of lipo-peroxidative process in hepatic and renal nuclear fractions of rats. Ind J Exp Biol 27, 449–452.Google Scholar
Diamante, C., Bergfeld, W.F., Belsito, D.V., Klaassen, C.D., Marks, J.G. Jr., Shank, R.C., Slaga, T.J., Synder, P.W. & Alan Andersen, F. (2009). Final report on the safety assessment of Basic Violet 1, Basic Violet 3 and Basic Violet 4. Int J Toxicol 28(6), 193S–204S.Google Scholar
ETAD (2007). Available at http://www.etad.com/information6htm (retrieved January, 2007).Google Scholar
Finney, D.J. (1971). Probit Analysis. London: Cambridge University Press.Google Scholar
Gill, T.S., Pande, J. & Tewari, H. (1991). Hemopathological changes associated with experimental aldicarb poisoning in fish (Puntius conchonius Hamilton). Bull Environ Contam Toxicol 47, 628–633.Google Scholar
Gill, T.S. & Pant, J.C. (1985). Erythrocytic and leukocytic responses to cadmium poisoning in a freshwater fish, Puntius conchonius Ham. Environ Res 36, 327–337.Google Scholar
Gill, T.S. & Pant, J.C. (1986). Chromatin condensation in the erythrocytes of fish following exposure to cadmium. Bull Environ Contam Toxicol 36, 199–203.Google Scholar
Giovannoni, M.P., Cesari, N., Vergelli, C., Graziano, A., Biancalani, C., Biagini, P., Ghelardini, C., Vivoli, E. & Piaz, V.D. (2007). 4-Amino-5-substituted-3(2H)-pyridazinones as orally active antiniciceptive agents: Synthesis and studies on the mechanism of action. J Med Chem 50, 3945–3953.Google Scholar
Goldberg, B. & Stern, A. (1977). The role of the superoxide anion as a toxic species in the erythrocyte. Arch Biochem Biophys 178, 218–225.Google Scholar
Gul, A., Yilmaz, M. & Isilak, Z. (2009). Acute toxicity of zinc sulphate (ZnSO4.H2O) (Poecilia reticulata P. 1859). GU J Sci 22, 59–65.Google Scholar
Halappa, R. & David, M. (2009). Behavioral responses of the freshwater fish, Cyprinus carpio (Linnaeus) following sublethal exposure to chlorpyrifos. Turk J Fish Aquat Sc 9, 233–238.Google Scholar
Hao, O.J., Kim, H. & Chiang, P.C. (2000). Decolorization of wastewater. Crit Rev Env Sci Tec 30, 449–505.Google Scholar
Khangrot, B.S. & Ray, P.K. (1990). Acute toxicity of toxic interaction of chromium and nickel to common guppy Poecilia reticulate (Peters). Bull Environ Contam Toxicol 44, 832–839.Google Scholar
Kori-Siakpere, O. & Ubogu, E.O. (2008). Sub lethal hematological effects of zinc on the freshwater fish, Heteroclarias sp. (Osteichthyes: Clariidae). Afr J Biotechnol 7, 2068–2073.Google Scholar
Koshino, I., Mohandas, N. & Takakuwa, Y. (2012). Identification of a novel role for dematin in regulating red cell membrane function by modulating spectrin–actin interaction. J Biol Chem 287, 35244–35250.Google Scholar
Lang, E., Qadri, S.M. & Lang, F. (2012). Killing me softly – suicidal erythrocyte death. Int J Biochem Cell Biol 44, 1236–1243.Google Scholar
Matsumoto, S.T., Mantovani, M.S., Malagutti, M.I.A., Dias, A.L., Fonseca, I.C. & Marin-Morales, M.A. (2006). Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish, Oreochromis niloticus and chromosome aberrations in onion root-tips. Genet Mol Biol 29, 148–158.Google Scholar
MOEF (2014). The gazette of india: extraordinary [part ii—sec. 3(i)], New Delhi, the 7th May, 2014. Available at http://www.moef.nic.in/sites/default/files/325.pdf (retrieved October, 2014).Google Scholar
Moss, J.A. & Hathway, D.E. (1964). Transport of organic compounds in the mammal partition of dieldrin and telodrin between the cellular components and proteins of blood. Biochem J 91, 384–393.Google Scholar
Nehls, S. & Segner, H. (2001). Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the comet assay. Environ Toxicol 16, 321–329.CrossRefGoogle ScholarPubMed
Ogata, H. & Murai, T. (1988). Changes in ammonia and amino acid levels in the erythrocytes and plasma of carp, Cyprinus carpio, during passage through the gills. J Fish Biol 33, 471–479.Google Scholar
Osman, M.M., EL-Fiky, S.A., Soheir, Y.M. & Abeer, A.I. (2009). Impact of water pollution on histopathological and eletrophoretic characters of Auriochromis niloticus fish. Res J Environ Toxicol 3, 9–23.Google Scholar
Pathan, T.S., Sonawane, D.L. & Khillare, Y.K. (2009). Toxicity and behavioral changes in freshwater fish, Rasbora daniconius exposed to paper mill effluent. Bot Res Int 2, 263–266.Google Scholar
Pretorius, E. & Kell, D.B. (2014). Diagnostic morphology: Biophysical indicators for iron-driven inflammatory diseases. Integr Biol (Camb) 6(5), 486–510.Google Scholar
Puvaneswari, N., Muthukrishnan, J. & Gunasekaran, P. (2006). Toxicity assessment and microbial degradation of azo dyes. Indian J Exp Biol 44, 618–626.Google Scholar
Robinson, T., McMullan, G., Marchant, R. & Nigam, P. (2001). Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77, 247–255.Google Scholar
Rojas, E., Valverde, M., Herrera, L.A., Altamirano, L.M. & Ostrosky, W.P. (1996). Genotoxicity of vanadium pentaoxide evaluate by the single cell electrophoresis assay in human lymphocytes. Mutat Res 359, 77–84.Google Scholar
Sawhney, A.K. & Johal, M.S. (2000). Erythrocyte alterations induced by malathion in Channa punctatus (Bloch). Bull Environ Contam Toxicol 64, 398–405.Google Scholar
Sengul, F. & Muezzinoglu, A. (1996). The strategies of ecological production. TTGV 130/S Final reports, Izmir.Google Scholar
Sweeney, A.E., Chipman, J.K. & Forsythe, S.J. (1994). Evidence for direct-acting oxidative genotoxiciy by reduction products of azo dyes. Environ Health Perspect 102(6), 119–122.Google Scholar
Timchalk, C., Nolan, R.J., Mendrala, A.L., Dittenber, D.A., Brzak, K.A. & Mattsson, J.L. (2002). A physiologically base pharmacokinetic and pharmacodynamic (PBPK/PD) model for the organophosphate insecticide chlorpyrifos in rats and humans. Toxicol Sci 66, 34–53.Google Scholar
Tonogai, Y., Ito, Y., Iwaida, M., Tati, M., Ose, Y. & Hori, M. (1979). Studies on the toxicity of coal-tar dyes III. J Toxicol Sci 5, 22–33.Google Scholar
Tonogai, Y., Ito, Y., Iwaida, M., Tati, M., Ose, Y. & Hori, M. (1980). Studies on the toxicity of coal-tar dyes III. J Toxicol Sci 5, 22–33.Google Scholar
Zeni, C., Bovolenta, M.R. & Stagni, A. (2002). Occurrence of echinocytosis in circulating RBC of black bullhead, Ictalurus melas (Rafinesque), following exposure to an anionic detergent at sublethal concentrations. Aquat Toxicol 57, 217–224.Google Scholar