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Environmental Scanning Electron Microscopy (ESEM) Study of Sea Urchin Embryos after Deciliation with Chloral Hydrate

Published online by Cambridge University Press:  02 July 2020

Heide Schatten  and
Amitabha Chakrabarti
Heide Schatten
Affiliation:
Dept. of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
Amitabha Chakrabarti
Affiliation:
Dept. of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
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Extract

Environmental Scanning Electron Microscopy has opened up the potential to study biological events in their native occurence. While still in the early stages of exploration, the ability to view samples in their native state with the resolution provided by scanning electron microscopy is highly desirable and will provide insights in dynamic processes which previously had only been possible by interpretation with indirect methods or by static images obtained with transmission electron microscopy.

In preliminary experiments to explore the potential of studying native events with the ESEM we have investigated sea urchin embryos after deciliation with chloral hydrate. The Philips XL-30 ESEM instrument was used to obtain images of lightly fixed (0.1% glutaraldehyde in sea water), lightly coated embryos that had been treated as follows. Chloral hydrate was added at a concentration of 0.1% to blastulae of Lytechinus pictus at 24 hours after fertilization. Observation with light microscopy indicates that cilia become gradually shed into the sea water within 10 to 20 hours after chloral hydrate application.

Type
Biological Ultrastructure/Microbiology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1130 - 1131
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Schatten, H., Chakrabarti, A., and Mitchell, K.Mol. Biol, of the Cell 8 (1997) p.52a.Google Scholar
2.Schatten, G., and Mazia, D.Exp. Cell Res. 98 (1976) 325–337.CrossRefGoogle Scholar
3. This work was supported in part by a grant from NASA (H.S.) and by funds from the University of Missouri-Columbia.Google Scholar