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Electrospinning Applications in Bioengineering: Fabrication of Bio-Engineered Skeletal Muscle

Published online by Cambridge University Press:  02 July 2020

T Telemeco ,
GL Bowlin ,
JA Matthews ,
NM Cohen ,
M Vida ,
GE Wnek ,
L Terracio  and
DG Simpson
T Telemeco
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
GL Bowlin
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
JA Matthews
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
NM Cohen
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
M Vida
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
GE Wnek
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
L Terracio
Affiliation:
The Dental School at New York University, New York, NY, 10010
DG Simpson
Affiliation:
Virginia Commonwealth University, Richmond, VA, 23298
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Abstract

The objective of this study is to develop a skeletal muscle prosthetic composed entirely of natural or otherwise bioresorbable materials that recapitulates the structure and function of intact muscle. in our fabrication strategy we begin by electrospinning (see Mathews et al., this volume) a hollow, cylindrical fascial sheath composed of poly-glycolic acid (PGA), poly-lactic acid (PLA), Type I collagen microfibers or a blend of these materials (figure 1). The resulting fascial sheath is a highly porous fabric composed of interwoven fibers that range from less than 1 micron to 2-5 microns in diameter (patents pending). Next, we isolate neonatal rat skeletal myoblasts by enzymatic dissociation. The dissociated tissue is subjected to an interval of differential adhesion to enrich the preparation in skeletal muscle myoblasts. The final cell pellet of myoblasts is then suspended at high density in Type I collagen. This suspension is polymerized at 37°C for 10-15 minutes and used to fill a fascial sheaths (patents pending).

Type
2001: A Space Odyssey: Biological and Materials Science Experiments Conducted in Real (Space) and Simulated (Bioreactors) Microgravity (Organized by D. Simpson and L. Terracio)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 144 - 145
Copyright
Copyright © Microscopy Society of America 2001

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