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Early Spreading and Propagation of Human Bone Marrow Stem Cells on Isotropic and Anisotropic Topographies of Silica Thin Films Produced via Microstamping

Published online by Cambridge University Press:  22 October 2010

A. Pelaez-Vargas ,
D. Gallego-Perez ,
N. Ferrell ,
M.H. Fernandes ,
D. Hansford  and
F.J. Monteiro
A. Pelaez-Vargas*
Affiliation:
INEB - Instituto de Engenharia Biomédica and Universidade do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA
D. Gallego-Perez
Affiliation:
Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA
N. Ferrell
Affiliation:
Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA
M.H. Fernandes
Affiliation:
Universidade do Porto, Faculdade de Medicina Dentária, Laboratório de Farmacologia e Biocompatibilidade, Porto, Portugal
D. Hansford
Affiliation:
Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA
F.J. Monteiro
Affiliation:
INEB - Instituto de Engenharia Biomédica and Universidade do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal
*
Corresponding author. E-mail: [email protected]
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Abstract

While there has been rapid development of microfabrication techniques to produce high-resolution surface modifications on a variety of materials in the last decade, there is still a strong need to produce novel alternatives to induce guided tissue regeneration on dental implants. High-resolution microscopy provides qualitative and quantitative techniques to study cellular guidance in the first stages of cell-material interactions. The purposes of this work were (1) to produce and characterize the surface topography of isotropic and anisotropic microfabricated silica thin films obtained by sol-gel processing, and (2) to compare the in vitro biological behavior of human bone marrow stem cells on these surfaces at early stages of adhesion and propagation. The results confirmed that a microstamping technique can be used to produce isotropic and anisotropic micropatterned silica coatings. Atomic force microscopy analysis was an adequate methodology to study in the same specimen the sintering derived contraction of the microfabricated coatings, using images obtained before and after thermal cycle. Hard micropatterned coatings induced a modulation in the early and late adhesion stages of cell-material and cell-cell interactions in a geometry-dependent manner (i.e., isotropic versus anisotropic), as it was clearly determined, using scanning electron and fluorescence microscopies.

Keywords

silica sol-gel filmssoft lithographymicrostampingcell adhesion and proliferationSEMAFM
Type
Special Section from Portugal Meeting
Information
Microscopy and Microanalysis , Volume 16 , Issue 6 , December 2010 , pp. 670 - 676
Copyright
Copyright © Microscopy Society of America 2010

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