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Differences in Chromatin Texture and Nuclear Fractal Dimension Between Hashimoto's and Lymphocytic Thyroiditis Lymphocytes

Published online by Cambridge University Press:  28 February 2019

Jasna Todorović ,
Marko Dinčić ,
Jelena Nešović Ostojić ,
Ivan Zaletel ,
Srdjan Lopičić ,
Duško Dundjerović ,
Svetislav Tatić ,
Sanjin Kovačević ,
Ivan Paunović  and
Nela Puškaš
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Jasna Todorović*
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Marko Dinčić*
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Jelena Nešović Ostojić
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Ivan Zaletel
Affiliation:
Faculty of Medicine, Institute of Histology and Embryology, University of Belgrade, Serbia
Srdjan Lopičić
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Duško Dundjerović
Affiliation:
Faculty of Medicine, Institute of Pathology, University of Belgrade, Serbia
Svetislav Tatić
Affiliation:
Faculty of Medicine, Institute of Pathology, University of Belgrade, Serbia
Sanjin Kovačević
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
Ivan Paunović
Affiliation:
Faculty of Medicine, Center for Endocrine Surgery, Clinical Center of Serbia, University of Belgrade, Serbia
Nela Puškaš
Affiliation:
Faculty of Medicine, Institute of Histology and Embryology, University of Belgrade, Serbia
Ljiljana Marković
Affiliation:
Faculty of Medicine, Institute of Pathological Physiology, University of Belgrade, Serbia
*
*Authors for correspondence: Todorović Jasna, E-mail: [email protected]; Dinčić Marko, E-mail: [email protected]
*Authors for correspondence: Todorović Jasna, E-mail: [email protected]; Dinčić Marko, E-mail: [email protected]
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Abstract

Previous evidence suggested that lymphocytic thyroiditis (LT) was a variant of Hashimoto's thyroiditis (HT), thus the aim of the current study is to quantify structural changes in histological specimens taken from HT and LT patients. A total of 600 images containing a single lymphocyte nucleus (300 nuclei per group) were obtained from 20 patients with HT and LT. In order to quantify changes in the nuclear architecture of investigated lymphocytes, the fractal dimension (FD) and some gray-level co-occurrence matrix texture parameters (angular second moment, inverse difference moment, contrast, entropy, and correlation) were calculated for each nucleus. A statistically significant difference in the FD of the “binary-outlined” nucleus and that of the corresponding “black-and-white” nucleus was detected between HT and LT lymphocyte nuclei. In addition, there was also a statistically significant difference in contrast and correlation between HT and LT lymphocyte nuclei. In conclusion, the results of this study suggested that there was a difference in structural complexity between investigated lymphocyte nuclei; additionally, LT lymphocytes possessed probably more complex texture and larger variations as well as more asymmetrical nuclei compared with HT lymphocytes. Accordingly, these findings indicate that LT is probably not a variant of HT; however, more complex studies are necessary to estimate differences between these types of thyroiditis.

Keywords

fractal dimensionGLCMHashimoto's thyroiditislymphocyte nucleilymphocytic thyroiditis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 3 , June 2019 , pp. 762 - 768
Copyright
Copyright © Microscopy Society of America 2019 

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