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Demonstration of Z-RNA in the Dog Eye Lens Epithelium (Germinative Zone)

Published online by Cambridge University Press:  02 July 2020

C.E. Gagna ,
J.H. Chen ,
H.R. Kuo  and
W.C. Lambert
C.E. Gagna
Affiliation:
Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey-Medical School, Newark, NJ07103
J.H. Chen
Affiliation:
Department of Biochemistry, New York University-Dental Center, New York, NY10010
H.R. Kuo
Affiliation:
Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey-Medical School, Newark, NJ07103
W.C. Lambert
Affiliation:
Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey-Medical School, Newark, NJ07103
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Extract

The purpose of this scientific investigation was to determine the presence and specific cellular localization of left-handed Z-RNA, within germinative zone (GZ) epithelium of the lens (Fig. 1), using anti-Z-RNA IgG polyclonal antibodies. Right-handed B-DNA has the ability to adopt the Z-DNA conformation in vitro (Sinden, 1994). Right-handed A-RNA can be transformed into Z-RNA under specific conditions (Hall et al., 1984), and Z-RNA has been identified in cultured cells (Zarling et al., 1990). Strong evidence supports the idea of Z-DNA in vivo (Sinden, 1994). Removal of proteins by fixatives can induce supercoiling which stabilizes Z-DNA (Sinden, 1994).

Anti-Z-RNA antibodies were produced in rabbits immunized with injections of Z-RNA: brominated-poly[ribosomal(G-C)]. For light microscopy, immunohistochemical studies (ABC method), normal dog lens tissues (1 yr old) were fixed in Carnoy's, embedded in paraffin and sectioned 2 μm thick. For electron microscopy (immunogold staining), pieces of epithelium from the GZ of normal dog lens (1 yr old) were fixed with 5% glutaraldehyde in 0.05 M phosphate buffer solution, pH 7.3.

Type
Cytochemistry (Light and Electron Histochemistry)
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1108 - 1109
Copyright
Copyright © Microscopy Society of America

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References

References:

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