Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T18:41:58.511Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytoskeleton-Organelle Interaction: Higher Plant Chloroplasts Are Contained In Actin Baskets And Attached To Actin Filaments And Bundles

Published online by Cambridge University Press:  02 July 2020

M.K. Kandasamy  and
R.B. Meagher
M.K. Kandasamy
Affiliation:
Department of Genetics, Life Sciences Building, University of Georgia, Athens, GA30606
R.B. Meagher
Affiliation:
Department of Genetics, Life Sciences Building, University of Georgia, Athens, GA30606
Get access

Extract

Plant organelles, including the dominant chloroplasts, migrate intracellularly on cytoplasmic strands (Fig. 1A-D). The chloroplasts in the leaf cells orient and redistribute in response to light to ensure maximum photosynthetic productivity. Their orderly distribution is also essential for proper transmission of organelle genome during cell proliferation. The movement and positioning of chloroplasts have been suggested to be mediated by the actin and tubulin-based cytoskeleton in green algae and higher plants. However, the actin structures controlling these processes have not been clearly delineated because of the difficulty in preserving and detecting the fine actin filaments in plant cells using conventional fixation methods and currently available antibodies.

We investigated the role of the actin cytoskeleton in the regulation of chloroplast movement and positioning by studying: 1) the structural relationships of microfilaments and chloroplasts in leaf cells of Arabidopsis; and 2) effects of an anti-actin drug, Latrunculin B (LAT-B), on intracellular distribution of chloroplasts.

Type
Low Temperature Methods for Immunolabeling of Cells and Tissues
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 296 - 297
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Williamson, R.E., Annu. Rev. Plant Physiol. Plant Mol. Biol. 44 (1993) 181–202.CrossRefGoogle Scholar
2.Lancelleet, S.A. al., Protoplasma 140(1987) 141–150.CrossRefGoogle Scholar
3.Kandasamy, M.K. et al., Plant J. 18 (1999) 681–691.CrossRefGoogle Scholar
4.Kandasamy, M.K. and Meagher, R.B., Cell Motil. Cytoskeleton 44 (1999) 110–118.3.0.CO;2-O>CrossRefGoogle Scholar
5. This research was supported by a grant from National Institute of Health (# GM36397-14Al).Dr. R. Cyr is acknowledged for providing an anti-tubulin polyclonal antibody.Google Scholar