Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-12-01T06:23:50.282Z Has data issue: false hasContentIssue false

Current Applications and Methods of Microvascular Corrosion Casting: A Review

Published online by Cambridge University Press:  02 July 2020

A. Lametschwandtner
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
H. Aichhorn
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
B. Minnich
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
Get access

Extract

Casting of hollow spaces with solidifying materials and subsequent removal of surrounding tissues by corrosive alkali and acids and the inspection of the remaining casts by bare eyes or the dissecting microscope is an old anatomical technique.

The introduction of polymerizing resins as casting materials which resulted in durable casts of even the smallest spaces (bile and blood capillaries) and the application of the scanning electron microscope with its high resolution and great depth of focus, enabled the qualitative and the quantitative analysis of the 3D-arrangement of tubular systems by means of their casts.

Presently, scanning electron microscopy of microvascular corrosion casts is used to study growing, stable or regressing blood vessel systems under physiological (e.g. during development, wound healing, metamorphosis) and pathological (e.g. tumor angiogenesis) conditions in qualitative and quantitative terms.

Type
Applications and Methods of Vascular Corrosion Casting—The 3-Dimensional Microvasculature of Tissues
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.J. Kus, . Folia Morph. (Wars) 28(1969) 134.Google Scholar
2.T. Murakami, . Arch, histol. jap. 32 (1971) 445.CrossRefGoogle Scholar
3.A.H. Aharinejad, and A. Lametschwandtner, , Eds., Microvascular corrosion casting in scanning electron microscopy. Techniques and applications. Springer, New York - Wien. (1992) 375.CrossRefGoogle Scholar
4.Motta, P.M. et al., Eds., Scanning electron microscopy of vascular casts: methods and applications. Kluwer Academic Publishers, Boston-Dordrecht-London (1992) 390.CrossRefGoogle Scholar