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Comparative Tissue Stainability of Lawsonia inermis (Henna) and Eosin as Counterstains to Hematoxylin in Brain Tissues

Published online by Cambridge University Press:  16 March 2015

Judith N. Alawa ,
Gbenga O. Gideon ,
Bamidele Adetiba  and
Clement B. Alawa
Judith N. Alawa*
Affiliation:
Department of Human Anatomy, Ahmadu Bello University, #56 Sokoto Zaria Road Samaru, Zaria 810271, Kaduna, Nigeria
Gbenga O. Gideon
Affiliation:
Department of Human Anatomy, Ahmadu Bello University, #56 Sokoto Zaria Road Samaru, Zaria 810271, Kaduna, Nigeria
Bamidele Adetiba
Affiliation:
Department of Human Anatomy, Ahmadu Bello University, #56 Sokoto Zaria Road Samaru, Zaria 810271, Kaduna, Nigeria
Clement B. Alawa
Affiliation:
National Animal Production and Research Institute, Ahmadu Bello University, Zaria-Sokoto Road Samaru, Zaria 810271, Kaduna, Nigeria
*
*Corresponding author. [email protected]
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Abstract

We hyposthesized that henna staining could provide an alternative to eosin when used as a counterstain to hematoxylin for understanding basic neurohistological principles. Therefore, this study was aimed at investigating the suitability of henna as counterstain to hematoxylin for the demonstration of the layer stratification and cellular distribution in the brain tissue. Henna stained nervous tissue by reacting with the basic elements in proteins via its amino groups. It stained the neuropil and connective tissue membranes brown and effectively outlined the perikarya of neurons with no visible nuclei demonstrating that it is an acidic dye. Henna as a counterstain to hematoxylin demonstrated reliability as a new neurohistological stain. It facilitated identification of cortical layer stratification and cellular distribution in brain tissue sections from Wistar rats. This was comparable to standard hematoxylin and eosin staining as morphological and morphometrical analyses of stained cells did not show significant differences in size or number. This study presents a method for staining with henna and demonstrates that although henna and eosin belong to different dye groups (anthraquinone and xanthenes, respectively) based on their chromophores, they share similar staining techniques and thus could be used interchangeably in neurohistology.

Keywords

lawsonia inermishennaneurohistologyhematoxylineosin
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 2 , April 2015 , pp. 343 - 350
Copyright
© Microscopy Society of America 2015 

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References

Avwioro, O.G. (2002). Histochemistry and Tissue Pathology, 1st ed. Nigeria: Claverianun press. pp. 134–213.Google Scholar
Badri, B.M. & Burkinshaw, S.M. (1993). Dyeing of wool and nylon 6.6 with henna and lawsone. Dyes Pigments 22(1), 15–25.CrossRefGoogle Scholar
Bassey, R.B., Bakare, A.A., Edagha, I.A., Osinubi, A.A.A. & Oremosu, A.A. (2012). Staining characteristics of Lonchocarpus cyanescens leaf extract on the testis of Sprague-Dawley rats. Microsc Microanal 18(4), 840–843.Google Scholar
Braide, W., Akobundu, C., Nwaogiekpe, R.N. & Njiribaeko, L.C. (2011). The use of extracts from four local Nigerian plants for the staining of selected bacteria and moulds. Afr J Biotechnol 5(1), 79–86.Google Scholar
Bruce-Gregorios, J.H. (1974). Histopathology Techniques, 2nd ed. Quezon city, USA: Goodwill Trading Company.Google Scholar
Cook, D.J. (2006). Staining Theory: Cellular Pathology, 2nd ed. United Kingdom: Scion Publishing Ltd. pp. 72–104.Google Scholar
Corbett, J.F. (1998). Hair Colorants: Chemistry and Toxicology. Dorset, UK: Micelle Press.Google Scholar
Crocker, J. & Burnett, D. (Eds.) (2005). The Science of Laboratory Diagnosis, 2nd ed. West Sussex, England: John Wiley and Sons Ltd.Google Scholar
Disbrey, B.D. & Rack, J.H. (1970). Histological Laboratory Methods. Edinburgh: Harcourt Brace/Churchill Livingstone.Google Scholar
Dweck, A.C. (2002). Natural ingredients for coloring and styling. J Cosmet Sci 24(5), 287–302.Google Scholar
Fessenden, R.J. & Fessenden, J.S. (Eds.) (1998). Organic Chemistry, 6th ed. Pacific Grove, CA: Brooks Cole Publishing Co.Google Scholar
Habbal, O.A., Al-Jabri, A.A. & El-Hag, A.G. (2007). Antimicrobial properties of Lawsonia inermis (henna): A review. Aust J Med Herbalism 19(3), 114–125.Google Scholar
Hikmat, U.J., Zabta, K.S. & Ashfaq, A.K. (2011). Staining effect of dye extracted from dry leaves of lawsonia inermis linn (henna) on angiospermic stem tissue. Pakistani J Bot 43(1), 383–389.Google Scholar
Jelly, R., Simon, W.L., Lennard, C., Kieran, F.L. & Almog, J. (2008). Lawsone: A novel reagent for the detection of latent fingermarks on paper surfaces. Chem Commun 5(8), 3513.Google Scholar
Khem, C., Jangid, B.L. & Rao, S.S. (2003). Henna: A potential source of non-farm employment and economic development in arid fringes. Agric Econ Res Rev 19 (Conference issue), 179.Google Scholar
Kirkland, D. & Marzin, D. (2003). An assessment of the genotoxicity of 2-hydroxy-1,4-naphthoquinone, the natural dye ingredient of Henna. Mut Res 537(2), 183–199.Google Scholar
Ling, E.A., Paterson, J.A., Privat, A., Mori, S. & Leblond, C.P. (1973). Investigation of glia cells in semithin sections, I. Identification of glial cells in the brain of young rats. J Comp Neur 149, 43–72.Google Scholar
Luna, L.G. (1960). Ed. Manual of Histologic and Special Staining Techniques. Ithaca, NY, USA: Cornell University, McGraw-Hill, Blakiston Division.Google Scholar
Marzin, D. & Kirkland, D. (2004). 2-Hydroxy-1,4-naphthoquinone, the natural dye of Henna, is non-genotoxic in the mouse bone marrow micronucleus test and does not produce oxidative DNA damage in Chinese hamster ovary cells. Mut Res 560(1), 41–47.Google Scholar
McMillan, D.C., Sarvate, S.D., Oatis, J.E. Jr & Jollow, D.J. (2004). Role of oxidant stress in lawsone-induced hemolytic anemia. Toxicol Sci 82(2), 647–655.Google Scholar
Ochei, J. & Kolhathar, A. (2005). Medical Laboratory Science: Theory and Practice. India: Tata McGraw Hill. (3rd reprint), 441pp.Google Scholar
Sturrock, R.R. (1976). Light microscopic identification of immature glial cells in semithin section of developing mouse corpus callosum. J Anatom 122, 531–537.Google ScholarPubMed
Upadhyay, B., Parveen, T.K., Dhaker, A.K. & Kumar, A. (2010). Ethnomedicinal and ethnopharmaco-statistical studies of Eastern Rajasthan, India. J Ethnopharmacol 129(1), 64–86.Google Scholar
Young, B. & Heath, J.W. (2000). Wheaters Functional Histology, 4th ed. USA: Elsevier, Churchill Livingstone.Google Scholar