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Comparative Study of the Measurement of Enamel Demineralization and Remineralization Using Transverse Microradiography and Electron Probe Microanalysis

Published online by Cambridge University Press:  24 April 2014

Nathan J. Cochrane ,
Youichi Iijima ,
Peiyan Shen ,
Yi Yuan ,
Glenn D. Walker ,
Coralie Reynolds ,
Colin M. MacRae ,
Nicholas C. Wilson ,
Geoffrey G. Adams  and
Eric C. Reynolds
Nathan J. Cochrane
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Youichi Iijima
Affiliation:
Department of Oral Health, Nagasaki University Graduate School of Biomedical Sciences, Unit of Social Medicine, 1-7-1 sakamoto, Nagasaki 852-8588, Japan
Peiyan Shen
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Yi Yuan
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Glenn D. Walker
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Coralie Reynolds
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Colin M. MacRae
Affiliation:
Microbeam Laboratory, CSIRO Process Science and Engineering, Bayview Avenue, Clayton, VIC 3168, Australia
Nicholas C. Wilson
Affiliation:
Microbeam Laboratory, CSIRO Process Science and Engineering, Bayview Avenue, Clayton, VIC 3168, Australia
Geoffrey G. Adams
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
Eric C. Reynolds*
Affiliation:
Oral Health CRC, Melbourne Dental School, Bio21 Institute, The University of Melbourne, 720 Swanston Street, VIC 3010, Australia
*
*Corresponding author.[email protected]
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Abstract

Transverse microradiography (TMR) and electron probe microanalysis (EPMA) are commonly used for characterizing dental tissues. TMR utilizes an approximately monochromatic X-ray beam to determine the mass attenuation of the sample, which is converted to volume percent mineral (vol%min). An EPMA stimulates the emission of characteristic X-rays from a variable volume of sample (dependent on density) to provide compositional information. The aim of this study was to compare the assessment of sound, demineralized, and remineralized enamel using both techniques. Human enamel samples were demineralized and a part of each was subsequently remineralized. The same line profile through each demineralized lesion was analyzed using TMR and EPMA to determine vol%min and wt% elemental composition and atomic concentration ratio information, respectively. The vol%min and wt% values determined by each technique were significantly correlated but the absolute values were not similar. This was attributable to the complex ultrastructural composition, the variable density of the samples analyzed, and the nonlinear interaction of the EPMA-generated X-rays. EPMA remains an important technique for obtaining atomic ratio information, but its limitations in determining absolute mineral content indicate that it should not be used in place of TMR for determining the mineral density of dental hard tissues.

Keywords

transverse microradiography (TMR)electron probe microanalyzer analysis (EPMA)remineralizationdemineralization
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 3 , June 2014 , pp. 937 - 945
Copyright
© Microscopy Society of America 2014 

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Footnotes

✠

We dedicate this work to the memory of Nathan J. Cochrane our friend and colleague. His dedication and passion for cariology was unmatched; his energy, enthusiasm and friendship will be sorely missed.

References

Ab-Ghani, Z., Ngo, H. & McIntyre, J. (2007). Effect of remineralization/demineralization cycles on mineral profiles of Fuji IX Fast in vitro using electron probe microanalysis. Aust Dent J 52, 276–281.Google Scholar
Angmar, B., Carlstrom, D. & Glas, J.E. (1963). Studies on the ultrastructure of dental enamel. IV. The mineralization of normal human enamel. J Ultrastruct Res 8, 12–23.Google Scholar
Baroni, C. & Marchionni, S. (2011). MIH supplementation strategies: Prospective clinical and laboratory trial. J Dent Res 90, 371–376.Google Scholar
Boyde, A., Switsur, V.R. & Fearnhead, R.W. (1961). Application of the scanning electron-probe X-ray microanalyser to dental tissues. J Ultrastruct Res 5, 201–207.Google Scholar
Cazaux, J. (2004). About the mechanisms of charging in EPMA, SEM, and ESEM with their time evolution. Microsc Microanal 10, 670–684.Google Scholar
Chu, J.S., Fox, J.L., Higuchi, W.I. & Nash, W.P. (1989). Electron probe micro-analysis for subsurface demineralization and remineralization of dental enamel. J Dent Res 68, 26–31.Google Scholar
Cochrane, N.J., Anderson, P., Davis, G.R., Adams, G.G., Stacey, M.A. & Reynolds, E.C. (2012). An X-ray microtomographic study of natural white-spot enamel lesions. J Dent Res 91, 185–191.Google Scholar
Cochrane, N.J., Cai, F., Huq, N.L., Burrow, M.F. & Reynolds, E.C. (2010). New approaches to enhanced remineralization of tooth enamel. J Dent Res 89, 1187–1197.Google Scholar
Cochrane, N.J., Saranathan, S., Cai, F., Cross, K.J. & Reynolds, E.C. (2008). Enamel subsurface lesion remineralisation with casein phosphopeptide stabilised solutions of calcium, phosphate and fluoride. Caries Res 42, 88–97.Google Scholar
Drouin, D., Couture, A.R., Joly, D., Tastet, X., Aimez, V. & Gauvin, R. (2007). CASINO V2.42: A fast and easy-to-use modeling tool for scanning electron microscopy and microanalysis users. Scanning 29, 92–101.Google Scholar
Drouin, D., Hovington, P. & Gauvin, R. (1997). CASINO: A new Monte Carlo code in C language for electron beam interactions—Part II: Tabulated values of the Mott cross section. Scanning 19, 20–28.Google Scholar
Edie, J.W. & Glick, P.L. (1980). Electron irradiation effects in the EPMA quantitation of organic specimens. Scan Electron Microsc, 271–284.Google Scholar
Frank, R.M., Capitant, M. & Goni, J. (1966). Electron probe studies of human enamel. J Dent Res 45, 672–682.Google Scholar
Heinrich, K.F.J. & Newbury, D.E. (1991). Electron Probe Quantitation. New York: Plenum Press.Google Scholar
Hovington, P., Drouin, D. & Gauvin, R. (1997). CASINO: A new Monte Carlo code in C language for electron beam interaction—Part I: Description of the program. Scanning 19, 1–14.Google Scholar
Landis, W.J. (1979). Application of electron probe X-ray microanalysis to calcification studies of bone and cartilage. Scan Electron Microsc 2, 555–570.Google Scholar
Ngo, H., Ruben, J., Arends, J., White, D., Mount, G.J., Peters, M.C., Faller, R.V. & Pfarrer, A. (1997). Electron probe microanalysis and transverse microradiography studies of artificial lesions in enamel and dentin: A comparative study. Adv Dent Res 11, 426–432.Google Scholar
Ngo, H.C., Mount, G., Mc Intyre, J., Tuisuva, J. & Von Doussa, R.J. (2006). Chemical exchange between glass-ionomer restorations and residual carious dentine in permanent molars: An in vitro study. J Dent 34, 608–613.Google Scholar
Pouchou, J.L. & Pichoir, F. (1984). A new model for quantitative X-ray microanalysis. Part I: Application to the analysis of homogeneous samples. Rech Aerosp 3, 13–38.Google Scholar
Pouchou, J.L. & Pichoir, F. (1991). Quantitative analysis of homogeneous or stratified microvolumes applying the model (PAP). In Electron Probe Quantification, Heinrich K.F.J. & Newbury D.E. (Eds.), pp. 31–76. New York: Plenum Press.Google Scholar
Selwitz, R.H., Ismail, A.I. & Pitts, N.B. (2007). Dental caries. Lancet 369, 51–59.Google Scholar
Sorbier, L., Rosenberg, E., Merlet, C. & Llovet, X. (1999). EPMA of porous media: A Monte Carlo approach. Microchimica Acta 132, 189–199.Google Scholar
Tanaka, K. & Iijima, Y. (2001). Acid resistance of human enamel in vitro after bicarbonate application during remineralization. J Dent 29, 421–426.CrossRefGoogle ScholarPubMed
Ten Bosch, J.J. & Angmar-Mansson, B. (1991). A review of quantitative methods for studies of mineral content of intra-oral caries lesions. J Dent Res 70, 2–14.Google Scholar
Ten Cate, J.M., Dundon, K.A., Vernon, P.G., Damato, F.A., Huntington, E., Exterkate, R.A., Wefel, J.S., Jordan, T., Stephen, K.W. & Roberts, A.J. (1996). Preparation and measurement of artificial enamel lesions, a four-laboratory ring test. Caries Res 30, 400–407.Google Scholar
Walker, G.D., Cai, F., Shen, P., Adams, G.G., Reynolds, C. & Reynolds, E.C. (2010). Casein phosphopeptide-amorphous calcium phosphate incorporated into sugar confections inhibits the progression of enamel subsurface lesions in situ . Caries Res 44, 33–40.Google Scholar
Wei, S.H. (1970). Electron microprobe analyses of the remineralization of enamel. J Dent Res 49, 621–625.CrossRefGoogle ScholarPubMed
Yanagisawa, T. & Miake, Y. (2003). High-resolution electron microscopy of enamel-crystal demineralization and remineralization in carious lesions. J Electron Microsc 52, 605–613.Google Scholar
Yip, H.K., Beeley, J.A. & Stevenson, A.G. (1995). Mineral content of the dentine remaining after chemomechanical caries removal. Caries Res 29, 111–117.Google Scholar