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Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography

Published online by Cambridge University Press:  02 July 2020

Tiffany A. Moisan ,
Mark Ellisman  and
Gina Sosinsky
Tiffany A. Moisan
Affiliation:
Scripps Institution of Oceanography, University of California, La Jolla, CA92093-0218
Mark Ellisman
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neurosciences and the San Diego Supercomputer Center, University of California, La Jolla, CA92093-0608.
Gina Sosinsky
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neurosciences and the San Diego Supercomputer Center, University of California, La Jolla, CA92093-0608.
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Extract

Understanding the light-harvesting properties of algae and higher plants are a fundamental topic in photosynthesis research. Much oceanographic research has focused on characterizing the in vivo chlorophyll-specific absorption coefficient, a*ph (λ) in phytoplankton because it serves as an input variable for bio-optical modeling of photosynthesis using remote sensing instrumentation such as moorings, drifters, and satellites. Values of a*ph (λ) vary spectrally and the magnitude depends on accessory pigments, photo-protective pigments, and pigment packaging effects. Several studies have shown that the contribution of cellular characteristics to a*ph(λ) varies with growth conditions including temperature, light, and nutrients. It has been shown that a*ph (λ) values in Phaeocystis vary predictably at 4°C over light intensities under light limitation. Phaeocystis demonstrated significant pigment package effects that depended on single cell diameter and thylakoid membrane stacking. Using thick sections obtained from fixed and embedded cultures of colonial P. antarctica, we calculated tomographic reconstructions of individual chloroplasts under light-limiting conditions for net photosynthesis in order to gain an understanding of the continuity of thylakoid membranes and understand the spatial relationship between the pyrenoid, the starch containing organelle, and thylakoid membranes.

Type
Ultrastructural Analysis Of Plant Cells
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1258 - 1259
Copyright
Copyright © Microscopy Society of America

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References

References:

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4.Acknowledgements: We thank S. Barlow and Y. Jones for technical assistance. Work was supported by NIH grant RR04050 (ME), ONR grant N00014-91-J-1186 (B.G. Mitchell), Southampton College, LIU and NASA Earth System Science Fellowship NGT 5-30036 to TAM.Google Scholar