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Characterization of Polymeric Microcapsules Containing a Low Molecular Weight Peptide for Controlled Release

Published online by Cambridge University Press:  29 January 2013

Keith Moore ,
Jennifer Amos ,
Jeffrey Davis ,
Robert Gourdie  and
Jay D. Potts
Keith Moore*
Affiliation:
Biomedical Engineering Program, University of South Carolina, Columbia, SC 29209, USA
Jennifer Amos
Affiliation:
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Jeffrey Davis
Affiliation:
Department of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC 29209, USA
Robert Gourdie
Affiliation:
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Jay D. Potts
Affiliation:
Biomedical Engineering Program, University of South Carolina, Columbia, SC 29209, USA Department of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC 29209, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

A need exists to prolong the release of rapidly metabolized peptides of a low molecular weight, while delivering this peptide without environmental interference. Previous studies have used bovine serum albumin (BSA) as a model peptide to study release characteristics from alginate microcapsules. BSA is 66 kDa in size, while the peptide of interest here, connexin-43 carboxyl-terminus mimetic peptide (αCT1), is only 3.4 kDa. Such a change in size results in a much different set of release parameters. Our overall goal is a sustained release over a 24+ h period. Prolonged application of the peptide to a wound site to investigate therapeutic effects is ideal. As a result, a diffusion method using alginate microcapsules, along with the addition of poly-l-lysine and poly-l-ornithine, has been explored. We first aimed to establish and characterize our parameters through a set of parametric tests. Variations in polymer coating, change in pH, and changes in loading ratio have previously been shown to effect release using model compounds. Here we test specific changes in these parameters to show effects on the release of αCT1. Additionally, the microcapsules were attached to several biomaterials and surgical implants by ultraviolet cross-linking to study the effectiveness of attachment and delivery. Analysis and measurements using phase contrast microscopy, scanning electron microscopy, and atomic force microscopy were used to characterize changes in microcapsule morphology.

Keywords

microencapsulationsodium alginateconnexin-43electrosprayingelectrohydrodynamicspoly-l-ornithinepoly-l-lysinezonula occludinsgap junctions
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 213 - 226
Copyright
Copyright © Microscopy Society of America 2013

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