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Characterization of Functionalized Multiwalled Carbon Nanotubes for Use in an Enzymatic Sensor

Published online by Cambridge University Press:  26 August 2014

Leonor Guadarrama-Fernández ,
Jorge Chanona-Pérez ,
Arturo Manzo-Robledo ,
Georgina Calderón-Domínguez ,
Adrián Martínez-Rivas ,
Jaime Ortiz-López  and
Jorge Roberto Vargas-García
Leonor Guadarrama-Fernández
Affiliation:
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Carpio S/N, Colonia Santo Tomas, CP 11340 México City, México
Jorge Chanona-Pérez*
Affiliation:
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Carpio S/N, Colonia Santo Tomas, CP 11340 México City, México
Arturo Manzo-Robledo
Affiliation:
Laboratorio de Electroquímica y Corrosión, Escuela Superior de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional, Edif. Z-5, 3er. Piso. UPALM-Zacatenco, CP 07738, México City, México
Georgina Calderón-Domínguez
Affiliation:
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Carpio S/N, Colonia Santo Tomas, CP 11340 México City, México
Adrián Martínez-Rivas
Affiliation:
Centro de Investigación en Computación, Instituto Politécnico Nacional, Av. Juan de Dios Bátiz s/n casi esquina Miguel Othón de Mendizábal, UPALM-Zacatenco, C.P. 07738, Del. Gustavo A. Madero, Mexico City, Mexico
Jaime Ortiz-López
Affiliation:
Escuela Superior de Físico Matemáticas, Instituto Politécnico Nacional, Edif. 9 UPALM-Zacatenco, CP 07738, México City, México
Jorge Roberto Vargas-García
Affiliation:
Escuela Superior de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional, Edif 7, UPALM-Zacatenco, CP 07738, México City, México
*
*Corresponding author. [email protected]
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Abstract

Carbon nanotubes (CNT) have proven to be materials with great potential for the construction of biosensors. Development of fast, simple, and low cost biosensors to follow reactions in bioprocesses, or to detect food contaminants such as toxins, chemical compounds, and microorganisms, is presently an important research topic. This report includes microscopy and spectroscopy to characterize raw and chemically modified multiwall carbon nanotubes (MWCNTs) synthesized by chemical vapor deposition with the intention of using them as the active transducer in bioprocessing sensors. MWCNT were simultaneously purified and functionalized by an acid mixture involving HNO3–H2SO4 and amyloglucosidase attached onto the chemically modified MWCNT surface. A 49.0% decrease in its enzymatic activity was observed. Raw, purified, and enzyme-modified MWCNTs were analyzed by scanning and transmission electron microscopy and Raman and X-ray photoelectron spectroscopy. These studies confirmed purification and functionalization of the CNTs. Finally, cyclic voltammetry electrochemistry was used for electrical characterization of CNTs, which showed promising results that can be useful for construction of electrochemical biosensors applied to biological areas.

Keywords

electron microscopyRaman spectroscopyXPSfunctionalized nanotubeselectrochemistrybiosensor
Type
Materials Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 5 , October 2014 , pp. 1479 - 1485
Copyright
© Microscopy Society of America 2014 

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