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Centrosome and Centriole Abnormalities During Cancer In the Transgenic Adenocarcinoma Mouse Prostate (Tramp) Model

Published online by Cambridge University Press:  02 July 2020

H. Schatten ,
A. Wiedemeier ,
M. Taylor ,
D. Lubahn ,
N. M. Greenberg ,
C. Besch-Williford ,
C. Rosenfeld ,
K. Day  and
M. Ripple
H. Schatten
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211 Cancer Research Center, Columbia, MO65211
A. Wiedemeier
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211 Cancer Research Center, Columbia, MO65211
M. Taylor
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
D. Lubahn
Affiliation:
Departments of Biochemistry and Child Health, University of Missouri-Columbia
N. M. Greenberg
Affiliation:
Department of Cell Biology and Scott Department of Urology, Baylor College of Medicine
C. Besch-Williford
Affiliation:
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO65211
C. Rosenfeld
Affiliation:
Departments of Biochemistry and Child Health, University of Missouri-Columbia
K. Day
Affiliation:
Departments of Biochemistry and Child Health, University of Missouri-Columbia
M. Ripple
Affiliation:
Institute on Aging, and Comprehensive Cancer Center, University of Wisconsin, and the William S. Middleton Veterans Administration Hospital, Madison, WI53792
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Extract

Centrosome abnormalities and abnormal mitotic formations have been shown in human breast cancer tissue and in LNCaP and DU145 prostate cancer cells in culture. Here we report on abnormal centrosome and centriole organization in the transgenic adenocarcinoma mouse prostate (TRAMP) model and on the implications for abnormal cell divisions, genomic instability, and apoptosis. Centrosomes are microtubule organizing centers which assemble bipolar spindles in normal cells. In cancer, centrosomes cause the formation of mono-, tri-, and multipolar mitoses which will separate chromosomes unequally during cell division.

We investigated the occurence of abnormal mitoses in TRAMP tissue in different stages of cancer progression using transmission electron microscopy (TEM). Advanced stages of prostate cancer are characterized by multiple abnormal mitoses ranging from tripolar to unusual multipolar formations. In addition, apoptotic bodies are abundant which may indicate that multipolar mitoses will lead to cell death or apoptosis.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1152 - 1153
Copyright
Copyright © Microscopy Society of America

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References

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