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Bringing Together High- and Low-Resolution Data: Electron Tomography of Budding Enveloped Alpha Virus

Published online by Cambridge University Press:  02 July 2020

R.H. Cheng ,
K. Hultenby ,
L. Haag ,
K. Forsell ,
H. Garoff ,
C.-E. Hsieh  and
M. Marko
R.H. Cheng
Affiliation:
Karolinska Institute, 14157Huddinge, Sweden
K. Hultenby
Affiliation:
Karolinska Institute, 14157Huddinge, Sweden
L. Haag
Affiliation:
Karolinska Institute, 14157Huddinge, Sweden
K. Forsell
Affiliation:
Karolinska Institute, 14157Huddinge, Sweden
H. Garoff
Affiliation:
Karolinska Institute, 14157Huddinge, Sweden
C.-E. Hsieh
Affiliation:
Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, Albany, NY, 12201-0509
M. Marko
Affiliation:
Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, Albany, NY, 12201-0509
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Abstract

The multi-layered architecture of alphaviruses is built up with the registration of nucleocapsid (NC) proteins and envelope spike proteins across a lipid bilayer. At the surface of the NC, capsid proteins are organized as 42 capsomers such that the three tails from each spike complex bind three capsomers. Alphaviruses have been extensively studied using cryo-EM and X-ray crystallography at near-atomic resolution (see review by Sedzik et al.). This work involves averaging images of many isolated virions, and makes use of the symmetry of the particle. The study of assembly and budding of virus particles at the cell surface, however, requires structural determination of individual virions in situ. Electron tomography offers a means for such studies.

Small features are obscured in typical thin-section (ca. 50nm) TEM micrographs due to the projection in 2D of all the structures throughout the section thickness. However, slices thinner than lnm can easily be obtained from tomographic reconstructions. Thus, small structures such as capsomers can be seen, even in thick (>100nm) specimens (Fig. 1b).

Type
Electron Tomography: Recent Advances and Applications (Organized by M. Marko)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 104 - 105
Copyright
Copyright © Microscopy Society of America 2001

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References

1.Sedzik, J.et al., Res. Dev. Virol., 3 (2001) in press.Google Scholar
2.Marko, M.et al., Proc. Microscopy & Microanalysis, (2000) 310.CrossRefGoogle Scholar
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5. Supported by NIH National Center for Research Resources (RR01219), Natural Science Research Council (NFR-11691), Medical Research Council (MFR-12175), Karolinska Institute, Tripep AB, and the Structural Biology Network in Sweden.Google Scholar