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An Integrated System for Transmission Electron Microscopy.

Published online by Cambridge University Press:  02 July 2020

B. Carragher ,
N. Kisseberth ,
D. Kriegman ,
R.A. Milligan ,
C.S. Potter ,
J. Pulokas  and
A. Reilein
B. Carragher
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
N. Kisseberth
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
D. Kriegman
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
R.A. Milligan
Affiliation:
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA92037
C.S. Potter
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
J. Pulokas
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
A. Reilein
Affiliation:
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Urbana, IL61801
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Extract

Macromolecular microscopy is becoming an increasingly important tool for structural biology. The development of improved capabilities for three-dimensional electron microscopy is critical for optimal progress in emerging integrative research in molecular cell biology. These techniques currently suffer from several severe disadvantages related to the tremendous time and effort required to acquire and analyze the data.

For several years we have been developing software for automated and intelligent acquisition of transmission electron micrographs [1,2,3]. Our overall goal is to develop a system for rapid and routine structure determination of macromolecular assemblies from specimens preserved in vitreous ice. Ultimately we plan to develop an integrated system that can produce an electron density map of a structure within a few hours of inserting a specimen into the electron microscope. With this goal in mind it is essential that the images be acquired using a digital camera rather than film.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 280 - 281
Copyright
Copyright © Microscopy Society of America

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References

1.Kisseberth, N., et al. (1998) JSB 120, 309–319.Google Scholar
2.Potter, C.S., et al. (1999) Ultramicroscopy, 77, 153–161.CrossRefGoogle Scholar
3.Pulokas, J., et al. (1999) JSB. 128, 250–256.Google Scholar
4.Ermantraut, E, et al. (1998) Ultramicroscopy 74 75–81.CrossRefGoogle Scholar
5.Koster, A. J. and de Ruijter, W. J. (1992) Ultramicroscopy, 40, 89–107.CrossRefGoogle Scholar
6. Support is provided by the NSF (9730056 and 9904547) and the IBM Shared University Research program.Google Scholar