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Advances in Microwave Assisted Specimen Processing. Benefits and Limitations Analyzed in Plant Nuclei and Drosophila Embryos in Vivo

Published online by Cambridge University Press:  02 July 2020

David M. Gartner  and
Mark A. Sanders
David M. Gartner
Affiliation:
Imaging Center, Department of Genetics, Cell Biology and Development 1445 Gortner Ave., College of Biological Sciences, University of Minnesota. , St. Paul, MN, 55108
Mark A. Sanders
Affiliation:
Imaging Center, Department of Genetics, Cell Biology and Development 1445 Gortner Ave., College of Biological Sciences, University of Minnesota. , St. Paul, MN, 55108
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Abstract

Recently, specimen processing using microwave irradiation was described as a potential tool to enhance a variety of procedures used in microscopy including fixations, embedding, antigen retrieval (AGR) and immunolabeling (1,2,3). A comparison of conventional staining and staining after microwave irradiation was performed for several fluorescent vital nuclear stains, Performing the labeling reaction under controlled microwave irradiation results in significant improvements in the labeling of nuclei, such as shortening the times of incubation and allowing the detection of otherwise inaccessible sites. In addition to investigate the basis of the enhancement, we have labeled intact samples such as living onion root meristems and Drosophila embryos. In both systems, vital fluorescent nuclear stains were applied to the specimen on a glass slide and was microwave irradiated using a variable wattage microwave (model 3451 Ted Pella, Inc.). This resulted in clear fluorescent labeling of the nuclei. This result has not been previously reported in the absence of microwave treatment, other than by microinjection.

Type
Biological Specimen Preparation/Cytochemistry/ Immunolabeling/Immunocytochemistry
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 468 - 469
Copyright
Copyright © Microscopy Society of America

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References

References:

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